Trifluralin-mediated polyploidization of Rosa chinensis minima (Sims) Voss seedlings

By D. Zlesak, C. Thill, N. Anderson. Euphytica, 2005

89 citations recorded by the source corpus.

Many diploid rose species and cultivars possess valuable traits that can be introgressed into modern tetraploid cultivars. Interspecific, interploidy crosses are possible, but triploid hybrids typically have limited fertility, hindering further breeding and selection. Tetraploidizing diploids before mating with tetraploids can alleviate fertility barriers. The efficiency of trifluralin was investigated for polyploidization of Rosa chinensis minima (2n = 2x = 14) seedlings. Treatments were trifluralin at 0.086% and 0.0086%, colchicine (0.5%), and distilled water and contained 2% dimethyl sulfoxide and a surfactant. Approximately 5 μl of the treatment solution was applied to the apical meristem of seedlings (N = 337, 82–85 per treatment) in the process of cotyledon expansion. Guard cell length, pollen diameter, and root tip squashes of rooted cuttings were used to detect polyploidy in meristematic layer (L)I, LII, and LIII, respectively. Trifluralin (0.086%) was the most effective treatment for polyploidization (LI 20.2%, LII 12.9%, LIII 12.9%), followed by trifluralin (0.0086%) (LI 10.6%, LII 7.1%, LIII 4.7%) and colchicine (LI 2.4%, LII 0%, LIII 0%). Polyploidization consistently occurred from LI inward. Polyploids as a group had reduced pollen stainability and a lower leaflet length to width ratio than diploids. In addition, two diploid seedlings were identified which produce 2n pollen. Considerations in selecting germplasm and generating somatically-induced polyploids from seedlings versus clones for use in breeding are discussed.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/844f3a66df8e7a29b569b4206ba7ec58978f43f7

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Citations in the corpus

Influence of ploidy level on morphology, growth and drought susceptibility in Spathiphyllum wallisii (2011, unknown): “…technique in many ornamentals, including Rhododendron, Alstroemeria, Syringa, Buddleja, Helleborus, Cyclamen, Rosa and Zantedeschia (Väinölä 2000; Rose et al. 2000a, b; Thao et al. 2003; Eeckhaut et al. 2004; Zlesak et al. 2005; Allum et al. 2007; Dhooghe et al. 2008; Khosravi et al. 2008).”
Mitotic chromosome doubling of plant tissues in vitro (2010, background): “In some recent publications, techniques are developed to unravel the genomic compilation of mixoploids and to study their formation (Zlesak et al. 2005).”