An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues

By M. Corces, Alexandro E. Trevino, Emily G. Hamilton, Peyton Greenside, Nicholas A. Sinnott-Armstrong, Sam Vesuna, Ansuman T. Satpathy, Adam J. Rubin, K. Montine, Beijing Wu and 12 more. Nature Methods, 2017

2,152 citations recorded by the source corpus.

We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol generates chromatin accessibility profiles from archival frozen tissue samples and 50-μm sections, revealing the activities of disease-associated DNA elements in distinct human brain structures. The Omni-ATAC protocol enables the interrogation of personal regulomes in tissue context and translational studies.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/83c80cb87796bcc3f51003e3b72e5005b693d73c

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Citations in the corpus

The chromatin accessibility landscape of primary human cancers (2018, background): “Recently, the advent of the assay for transposaseaccessible chromatin using sequencing (ATAC-seq) (16) has enabled the genome-wide profiling of chromatin accessibility in small quantities of frozen tissue (17).”
Chromatin Potential Identified by Shared Single-Cell Profiling of RNA and Chromatin (2020, methodology): “A single nucleus suspension was prepared following the OMNI-ATAC protocol (Corces et al., 2017).”
Identification of transcription factor binding sites using ATAC-seq (2018, positive): “ATAC-seq data K562 from 50.000 cells from [14] and combined single cell ATAC-seq data from H1-ESC and K562 cells [33] and Omni-ATAC K562 cells from [6].”