Rapid Detection and Quantification of RNA of Ebola and Marburg Viruses, Lassa Virus, Crimean-Congo Hemorrhagic Fever Virus, Rift Valley Fever Virus, Dengue Virus, and Yellow Fever Virus by Real-Time…

By Christian Drosten, S. Göttig, S. Schilling, M. Asper, M. Panning, H. Schmitz, S. Günther. Journal of Clinical Microbiology, 2002

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Crimean-Congo hemorrhagic fever. (2004, methodology): “Drosten et al. (2002a)developed one-step real-time RT-PCR assay for detecting CCHF ng primers to the nucleoprotein gene; however, they use NA-intercalating dye, SybrGreen I, for detecting the P roduct because no conserved binding site for a 5′-nuclease robe could be found.”
Zika virus infection complicated by Guillain-Barre syndrome--case report, French Polynesia, December 2013. (2014, methodology): “org ELISA, ALERE Australia) and reverse transcription-polymerase chain reaction (RT-PCR) [2], and ZIKA by RT-PCR [3], were negative on blood samples eight days after the beginning of influenza-like symptoms (corresponding to Day 1), prior to the administration of intravenous immunoglobulin.”