Isolation, identification and characterization of a feather degrading bacterium.
By S. G. Joshi, M. Tejashwini, N. Revati., R. Sridevi, D. Roma. 2007
174 citations recorded by the source corpus.
Feather constitutes over 90% protein, the main component being beta-keratin, a fibrous and insoluble structural protein extensively cross linked by disulfide bonds. This renders them resistant to digestion by animal, insects and proteases leading to serious disposal problems. In addition to this, feather waste is produced at the rate of 22 million kg per year (US alone). Use of keratinolytic microorganisms for feather degradation is an economical, environmental friendly alternative. Keratinases which are produced by these keratinolytic organisms could be used to degrade feather waste and further the digested products could be an excellent material for producing animal feed, fertilizers or natural gas. A feather-degrading bacterium was isolated from poultry waste. This bacterium was grown in basal media with feathers as its primary source of carbon, nitrogen, sulfur and energy. The organism is rod shaped, highly motile, endospore forming, catalase positive and gram negative. Phenotypic characterization carried out in our laboratory showed that this novel gram negative bacterium belongs to Bacillus genus. The organism is designated as Bacillus and named as Bacillus sp PW-1. The isolated strain has activity of 50 U/ml. This novel keratinolytic isolate could be a potential candidate for degradation and utilization of feather keratin.
Subfields: Biotechnology, Genetics, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/83859700111dee2efea0f55270dd21aa308c0624
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Citations in the corpus
- Microbial keratinases and their prospective applications: an overview (2006, background): “This can be exemplified from the literature where keratinase is mainly produced during the late exponential or stationary phase of microbial growth (Williams et al. 1990; Cheng et al. 1995; Sangali and Brandelli 2000; Vidal et al. 2000; Kim et al. 2001; Ramnani and Gupta 2004; Thys et al. 2004),…”
- Green synthesis of silver nanoparticles using keratinase obtained from a strain of Bacillus safensis LAU 13 (2015, methodology): “7 U ml at 120 h, which is similar to KA reported for some bacilli [17, 31].”