Microbial degradation of lignin: Role of lignin peroxidase, manganese peroxidase, and laccase

By T. Higuchi. Proceedings of the Japan Academy. Series B, Physical and biological sciences, 2004

158 citations recorded by the source corpus.

Lignin peroxidase (LiP), laccase (LA) and manganese peroxidase (MnP) of white-rot basidiomycetes such as Phanerochaete chrysosporium, Coliorus versicolor, Phlebia radiata and Pleurotus eryngii catalyze oxidative degradation of lignin substructure model compounds and synthetic lignins (DHPs). Side chain- and aromatic ring cleavage products of both phenolic and non-phenolic substrates oxidized by LiP were isolated and characterized by NMR and MS. The cleavage mechanism was elucidated by using 18O, 2H, and 13C labeled lignin substructure dimers with 18O2 and H2 18O. Recent studies suggested that LiP is capable of oxidizing lignin directly at the protein surface via a long-range electron transfer process. LA and MnP, which oxidize phenolic but not non-phenolic moieties, generally degrade lignin stepwise from phenolic moieties. However, recent studies indicated that MnP and LA can degrade both phenolic and non-phenolic aromatic moieties of lignin with some special mediators.

Subfields: Biomedical Engineering, Biotechnology, Plant Science

View Paper: https://www.semanticscholar.org/paper/824725ca7ad07cab5170e934405fb0a828f5d806

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biomedical Engineering

In Biotechnology

In Plant Science

Citations in the corpus

Biological pretreatment of lignocelluloses with white-rot fungi and its applications: A review (2011, methodology): “The fungi have been studied in connection with several ligninolytic enzymes, such as lignin peroxidases (LiP), manganese peroxidases (MnP), laccase (Lac), and versatile peroxidases (VP) (Higuchi 2004; Wong 2009).”
Phenolic acids act as signaling molecules in plant-microbe symbioses (2010, background): “Lignin degradation by the white rot fungi is a complex process mediated by the action of several extra cellular enzymes, of which lignin peroxidases are the most important.(60) Ligninolytic micro-organisms are important in agriculture and industry with a wide tolerance of temperature, pH, oxygen lim”
Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota: A review (2010, background): “The yellow laccase was suggested to be formed as a result of blue laccase modification by products of lignin degradation, which might play a role as natural electron-transfer mediators for the oxidation of non-phenolic substances (Higuchi, 2004).”
Lignin Biodegradation with Laccase-Mediator Systems (2014, background): “They are involved in both lignin biosynthesis and lignin degradation, pigment formation in fungal spores, plant pathogenesis, and as fungal virulence factors, in iron metabolism and kernel browning processes in plants (Hood et al., 2003; Higuchi, 2004; Hoopes and Dean, 2004; Zhu and Williamson, 2004”
Paper pulp delignification using laccase and natural mediators (2007, methodology): “…25 September 2006 bstract Three plant phenols, namely acetosyringone, syringaldehyde and p-coumaric acid, were selected as laccase redox mediators to investigate the nzymatic delignification of paper pulp (obtained from kraft cooking of eucalypt wood) in combination with peroxide bleaching.”
Degradation and transformation of humic substances by saprotrophic fungi: processes and mechanisms (2007, background): “The role of the nonspecific oxidizing enzymes in HS transformation Most of the basic research into extracellular nonspecific oxidizing enzymes in white rot fungi has focused on their role in lignin degradation (Higuchi 2004; Kirk & Farrell 1987).”