Cell disruption for microalgae biorefineries.

By Emre Günerken, E. D’Hondt, M. Eppink, L. Garcia-Gonzalez, K. Elst, R. Wijffels. Biotechnology Advances, 2015

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Lipid recovery from wet oleaginous microbial biomass for biofuel production: A critical review (2016, background): “HPH is currently used in industry for high-value protein recovery because it is simple to operate and scale up [24,112].”
Microalgae Potential and Multiple Roles—Current Progress and Future Prospects—An Overview (2016, methodology): “[50] reported the lack of very high disruption efficiency in ultra-sonication method.”
Apparent digestibility of nutrients, energy, essential amino acids and fatty acids of juvenile Atlantic salmon (Salmo salar L.) diets containing whole-cell or cell-ruptured Chlorella vulgaris meals at five dietary inclusion levels (2017, methodology): “…performance of farmed Atlantic salmon and this area warrants further 465 investigation.466 Many different methods have been used for disruption of C. vulgaris cell walls with varying degrees of 467 success and realistic potential for industrial scale-up (Safi et al. 2014a; Günerken et al. 2015).”
Electrotechnologies applied to microalgal biotechnology – Applications, techniques and future trends (2018, background): “The bulk temperature during the treatment may result in the degradation of labile compounds including the ones inside the cells [139,140], but also contribute to an increased extraction of lipids [140–142] and proteins [143].”