Clonogenic assay of cells in vitro

By N. A. Franken, H. Rodermond, J. Stap, J. Haveman, C. Bree. Nature Protocols, 2006

4,030 citations recorded by the source corpus.

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

RNF168 binds and amplifies ubiquitin conjugates on damaged chromosomes to allow accumulation of repair proteins. (2009, neutral): “radiation dose. Comparison of the survival curves for different treatments was performed as described ( Franken et al., 2006 ).”
ColonyArea: An ImageJ Plugin to Automatically Quantify Colony Formation in Clonogenic Assays (2014, methodology): “Cells are usually identified by staining with a crystal violet dye [3], which primarily binds to polyanionic sugar molecules such as DNA in the nucleus of mammalian cells [8].”
In Vitro Tumor Models: Advantages, Disadvantages, Variables, and Selecting the Right Platform (2016, methodology): “The colony formation assay is used to measure the ability of a single cell to grow into a colony and is used to assess clonogenic survival (Franken et al., 2006; Hirschhaeuser et al., 2010).”
Mammalian Cell Viability (2011, methodology): “Finally, it should be noted that PS exposure can occur on cells for phagocytic removal independent of apoptosis (3) and that PS exposure can be compromised in cells in which autophagy is impaired (4).”