Circular RNA circMTO1 acts as the sponge of microRNA‐9 to suppress hepatocellular carcinoma progression
By Dan Han, Jiangxue Li, Huamin Wang, X. Su, J. Hou, Yan Gu, C. Qian, Yun Lin, Xiang Liu, Mingyan Huang and 4 more. Hepatology, 2017
1,056 citations recorded by the source corpus.
Noncoding RNAs play important roles in cancer biology, providing potential targets for cancer intervention. As a new class of endogenous noncoding RNAs, circular RNAs (circRNAs) have been recently identified in cell development and function, and certain types of pathological responses, generally acting as a microRNA (miRNA) sponge to regulate gene expression. Identifying the deregulated circRNAs and their roles in cancer has attracted much attention. However, the expression profile and function of circRNAs in human hepatocellular carcinoma (HCC) remain to be investigated. Here, we analyzed the expression profile of human circRNAs in HCC tissues and identified circMTO1 (mitochondrial translation optimization 1 homologue; hsa_circRNA_0007874/hsa_circRNA_104135) as one circRNA significantly down‐regulated in HCC tissues. HCC patients with low circMTO1 expression had shortened survival. By using a biotin‐labeled circMTO1 probe to perform RNA in vivo precipitation in HCC cells, we identified miR‐9 as the circMTO1‐associated miRNA. Furthermore, silencing of circMTO1 in HCC could down‐regulate p21, the target of oncogenic miR‐9, resulting in the promotion of HCC cell proliferation and invasion. In addition, the tumor‐promoting effect of circMTO1 silencing was blocked by miR9 inhibitor. Intratumoral administration of cholesterol‐conjugated circMTO1 small interfering RNA promoted tumor growth in HCC‐bearing mice in vivo. Conclusion: circMTO1 suppresses HCC progression by acting as the sponge of oncogenic miR‐9 to promote p21 expression, suggesting that circMTO1 is a potential target in HCC treatment. The decrease of circMTO1 in HCC tissues may serve as a prognosis predictor for poor survival of patients. (Hepatology 2017;66:1151‐1164).
Subfields: Cancer Research, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/81755e5f160be1b24d754898882234c408bba0c4
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Citations in the corpus
- Circular RNA and its mechanisms in disease: From the bench to the clinic. (2018, background): “In addition, HCC patients with reduced circMTO1 expression showed decreased survival, suggesting that circMTO1 might be a potential target in HCC therapy (Han et al., 2017).”
- Circular RNA cSMARCA5 inhibits growth and metastasis in hepatocellular carcinoma. (2018, background): “In fact, many studies have proved that circRNAs can function as sponges for microRNAs (miRNAs) [13-20] or bind to proteins[21, 22], and altered circRNA levels can result in aberrant expression of gene products that may contribute to cancer”
- Noncoding RNA:RNA Regulatory Networks in Cancer (2018, background): “Conversely, circMTO1 was identified as a downregulated circRNA in HCC, and it functions as a tumor suppressor by sponging miR-9 to upregulate p21 and suppress HCC development [163].”