Effect of probiotic Bacillus subtilis Ch9 for grass carp, Ctenopharyngodon idella (Valenciennes, 1844), on growth performance, digestive enzyme activities and intestinal microflora

By Zhixin Wu, X. Feng, L. Xie, X. Peng, J. Yuan, Xiaoxuan Chen. 2012

111 citations recorded by the source corpus.

Summary In the present study, Bacillus subtilis Ch9 was evaluated as a probiotic in grass carp, Ctenopharyngodon idella (Valenciennes, 1844). For 56 days the grass carp (50 ± 2.5 g) were given a feed containing B. subtilis Ch9 in three concentrations: 1.0 × 109 (T1), 3.0 × 109 (T2) and 5.0 × 109 (T3) CFU kg−1 feed in triplicate treatments. The control group (T0) was given feed without B. subtilis Ch9 for the same period. Determined were the specific growth rate (SGR), feed conversion ratio (FCR), and digestive enzyme activities in the intestine and hepatopancreas as well as the intestinal microflora. After 56 days, fish receiving the diets supplemented with B. subtilis Ch9 showed significantly higher SGR and lower FCR (P 0.05) by the dietary treatments. From days 14 to 56 of the experiment, higher protease, amylase and lipase activities in the foregut, midgut hindgut and hepatopancreas were observed in T1, T2 and T3 (P 0.05), but the ratio of Bacillus was significantly higher (P < 0.05) than in control fish. The total anaerobic bacterial quantity, Bifidobacterium and Lactobacillus were significantly higher (P < 0.05) in fish fed B. subtilis Ch9 compared with fish fed control feed. In conclusion, an optimum dose of B. subtilis Ch9 could modulate intestinal microflora, induce digestive enzyme activity and potentially promote the digestion and absorption of nutrients, as well as improve the growth performance of grass carp significantly.

Subfields: Aquatic Science, Immunology

View Paper: https://www.semanticscholar.org/paper/816982f37b154ba8eb15179850e3359349bc10a7

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Citations in the corpus

Host-Associated Probiotics: A Key Factor in Sustainable Aquaculture (2020, background): “In a study with grass carp (Ctenopharyngodon idella), Wu et al. (2012) revealed that dietary administration of B. subtilis isolated from grass carp significantly improved specific growth rate, feed conversion ratio, enzyme activity, and total bacteria counts in the GI tract of the fish.”
Genus bacillus, promising probiotics in aquaculture: Aquatic animal origin, bio-active components, bioremediation and efficacy in fish and shellfish (2019, background): “Wu et al. (2012) fed grass carp with different levels (1.0 109, 3.0 109, and 5.0 109 CFU kg 1) of another strain of B. subtilis (B. subtilis Ch9) and found that probitoic fed fish had significantly improved weight gain, SGR and FCR, with the best results achieved when fish were fed with 3.0 109 CFU…”
Modulation of nutrient digestibility and digestive enzyme activities in aquatic animals: The functional feed additives scenario (2017, background): “Wu et al. (2012) reported that amylase activity in the FG of grass carp increased significantly from day 14 to 56 when fish were fed 3.0 9 109 B. subtilis.”