Characterization of Candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity
By Yang Yong, Yongxiao Bai, Yan-feng Li, Lei Lin, Yanjun Cui, Chungu Xia. 2008
140 citations recorded by the source corpus.
Abstract Magnetic microspheres were prepared by suspension polymerization of glycidyl methacrylate, ethylene glycol dimethacrylate and vinyl acetate in the presence of oleic acid-coated magnetite, and then, alcoholysed to improve their hydrophilicity. Candida rugosa lipase was covalently immobilized on the hydrophilic magnetic microspheres via the active epoxy groups with the activity yield up to 64.2%. The resulting immobilized lipase had better resistance to pH and temperature inactivation in comparison to free lipase, the adaptive pH and temperature ranges of lipase were widened, and it exhibited good thermal stability and reusability. The resistance of immobilized lipase against urea denaturation was also improved, and the immobilized lipase retained 40% of the activity in the presence of 5 M urea whereas free enzyme retained only 8% of activity. Kinetic parameters were also determined for both immobilized and free lipase. The K m value for immobilized (1.03 mg/ml) was higher than that of the free lipase (0.52 mg/ml), whereas the V max value was smaller for the immobilized lipase.
Subfields: Electrical and Electronic Engineering, Molecular Biology, Spectroscopy
View Paper: https://www.semanticscholar.org/paper/7f87627dd9bebb0e185a60829b73942ed0fd8375
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Citations in the corpus
- Enzyme Immobilization: An Overview on Nanoparticles as ImmobilizationMatrix (2015, background): “Besides, natural polymers various synthetic polymeric materials are also used as support as they possess good mechanical stability, moreover they can be modified easily [49,50].”
- Application of magnetic chitosan composites for the removal of toxic metal and dyes from aqueous solutions. (2013, background): “Furthermore, by chemically modifying the polymeric shells of these magnetic composites, the functionality can be tailored for various applications in diverse fields such as high-density data storage, magnetic resonance imaging (MRI), drug delivery, therapy, diagnosis, bioseparation, and enzyme immob”