Experimental interrogation of the path dependence and stochasticity of protein evolution using phage-assisted continuous evolution

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Epistasis in protein evolution (2016, background): “In such cases, evolutionary processes exhibit strong stochasticity in their outcomes: parallel populations evolving under the same dynamics will reach different endpoints in response to some selective pressure, because the permissive substitutions that happen to fix will generally be different in ea”
In Vivo Continuous Directed Evolution (2014, unknown): “This method has since been expanded to address the effects of varying levels of mutagenesis and selection stringency on enzyme evolution [22], the path dependence of convergent protein evolution [23], the effects of neutral drift, negative selection, and stringency modulation on enzyme evolution [24”
Negative selection and stringency modulation in phage-assisted constinuous evolution (2014, unknown): “1,6,7 The successful evolution of activity on P T3 instead required initial selection on a hybrid T7/T3 promoter (P T7/T3 ) that served as an evolutionary stepping-stone toward the P T3 selection.”