Appropriate pCO2 treatments in ocean acidification experiments

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Effects of ocean acidification on hatch size and larval growth of walleye pollock (Theragra chalcogramma) (2013, background): “Midwater pelagic areas are more physiochemically stable than shallow coastal waters which can exhibit significant natural, short-term variation in CO2 levels (Hofmann et al., 2011; McElhany and Busch, 2012).”
Offspring sensitivity to ocean acidification changes seasonally in a coastal marine fish (2014, background): “As a result, contempo rary coastal organisms already experience a wide range of pH and CO2 conditions, most of which are not predicted to occur in the open ocean for hundreds of years—if ever (Cai et al. 2011, Melzner et al. 2012).”
The influence of food supply on the response of Olympia oyster larvae to ocean acidification (2013, background): “The 5 accompanying seawater high pCO 2 target was 1000 µatm, a realistic and appropriate level designed to represent a doubling of the approximate current pCO 2 level during summer months in our study region (Moss et al., 2010; McElhany and Busch, 2013).”