Enzyme promiscuity: a mechanistic and evolutionary perspective.

By O. Khersonsky, Dan S. Tawfik. Annual Review of Biochemistry, 2010

1,292 citations recorded by the source corpus.

Subfields: Biochemistry, Materials Chemistry, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/7d7455f9126ac2834928831cbcc4f75e3e44bc9f

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In Biochemistry

Frances H. Arnold

In Materials Chemistry

In Molecular Biology

Citations in the corpus

Machine-learning-guided directed evolution for protein engineering (2018, background): “Directed evolution finds local optima through repeated local searches, taking advantage of functional promiscuity and the clustering of functional sequences in sequence space 5,7 (Fig.”
The Statistical Trends of Protein Evolution: A Lesson from AlphaFold Database (2022, neutral): “…with the experimental observations that ancestral enzymes are likely to have high promiscuity, as they may be able to catalyze a wide variety of chemical reactions (O’Loughlin et al. 2006; Khersonsky and Tawfik 2010; Takano et al. 2013; Petrovic et al. 2018; Gardner et al. 2020; Modi et al. 2021).”
Constraint-based models predict metabolic and associated cellular functions (2014, background): “It is thought that ancestral enzymes were promiscuous and inefficient, and that they have evolved to become catalytically efficient and specific 43 .”
Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries (2013, background): “Therefore, the YESS system provides a means to address and overcome a major issue in protease engineering, and in directed evolution in general, which is that overwhelmingly, mutations that increase the catalytic activity toward a desired substrate also result in relaxed specificity or higher cataly”
Probing the Mutational Interplay between Primary and Promiscuous Protein Functions: A Computational-Experimental Approach (2012, background): “Nevertheless, many proteins possess weak promiscuous functions, which are more or less related to the primary activity, but involve different substrates or different chemical alterations [1–5].”