The Cold-Inducible CBF1 Factor–Dependent Signaling Pathway Modulates the Accumulation of the Growth-Repressing DELLA Proteins via Its Effect on Gibberellin Metabolism[W]

By P. Achard, F. Gong, Soizic Cheminant, Malek Alioua, P. Hedden, P. Genschik. The Plant Cell Online, 2008

742 citations recorded by the source corpus.

Plants have evolved robust mechanisms to respond and adapt to unfavorable environmental conditions, such as low temperature. The C-repeat/drought-responsive element binding factor CBF1/DREB1b gene encodes a transcriptional activator transiently induced by cold that controls the expression of a set of genes responding to low temperature (the CBF regulon). Constitutive expression of CBF1 confers freezing tolerance but also slows growth. Here, we propose that low temperature–induced CBF1 expression restrains growth at least in part by allowing the accumulation of DELLAs, a family of nuclear growth-repressing proteins, the degradation of which is stimulated by gibberellin (GA). We show that cold/CBF1 enhances the accumulation of a green fluorescent protein (GFP)–tagged DELLA protein (GFP-RGA) by reducing GA content through stimulating expression of GA-inactivating GA 2-oxidase genes. Accordingly, transgenic plants that constitutively express CBF1 accumulate less bioactive GA and as a consequence exhibit dwarfism and late flowering. Both phenotypes are suppressed when CBF1 is expressed in a line lacking two DELLA proteins, GA-INSENSITIVE and REPRESSOR OF GA1-3. In addition, we show that DELLAs contribute significantly to CBF1-induced cold acclimation and freezing tolerance by a mechanism that is distinct from the CBF regulon. We conclude that DELLAs are components of the CBF1-mediated cold stress response.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/7c3eb3d819d1599f0b5489e1cb9e0a4edc1ae62d

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Citations in the corpus

Integrated phytohormone production by the plant growth-promoting rhizobacterium Bacillus tequilensis SSB07 induced thermotolerance in soybean (2019, background): “Supplemental data for this article can be accessed at https://doi.org/10.1080/17429145.2019.1640294 GAs function antagonistically, and the endogenous levels of both GAs and auxin are downregulated, eventually resulting in dwarfism (Achard et al. 2008; Miura and Furumoto 2013).”
Analysis of Cytokinin Mutants and Regulation of Cytokinin Metabolic Genes Reveals Important Regulatory Roles of Cytokinins in Drought, Salt and Abscisic Acid Responses, and Abscisic Acid Biosynthesis[C][W] (2011, background): “The morphological adjustment that optimizes growth in response to environmental cues was shown to be an important mechanism of plant survival (Sharp et al., 2004; Achard et al., 2006; Achard et al., 2008).”
The role of gibberellin signalling in plant responses to abiotic stress (2014, background): “GA signalling and stress tolerance Many of the studies linking DELLA function to restricted growth under abiotic stress also showed a positive effect on stress tolerance (Achard et al., 2006; Achard et al., 2008b; Fukao and Bailey-Serres, 2008b; Magome et al., 2004).”
Advances in physiological and molecular aspects of plant cold tolerance (2017, positive): “Achard et al. (2008) investigated the relationship between CBF1 and GA pathways and indicated that constitutive expression of CBF1 leads to less bioactivity of GA resulting in dwarfism in plants and delay in flowering.”
Molecular Basis of Plant Cold Acclimation: Insights Gained from Studying the CBF Cold Response Pathway1 (2010, background): “However, a more profound reason for the observed effects of CBF expression was discovered by Achard et al. (2008a).”
Gibberellin signaling in plants (2013, background): “contributes to plant fitness during environmental stress (Achard et al., 2008; Wild et al., 2012).”
Drought, salt, and temperature stress-induced metabolic rearrangements and regulatory networks (2012, background): “…et al., 2000, 2004) and CBF/DREB1 overexpressing plants accumulated higher levels of proline and soluble sugars (glucose, fructose, sucrose, and raffinose) when grown under normal growth conditions and during cold acclimation (Gilmour et al., 2000, 2004; Cook et al., 2004; Achard et al., 2008).”
Temperature regulation of plant phenological development (2015, background): “High GA-2-oxidase activity, thus, reduces GA content and restrains growth rate in the vegetative tissues (Achard et al., 2008; Achard and Genschik, 2009).”
Plant abiotic stress response and nutrient use efficiency (2020, background): “restrains growth and enhances freezing tolerance (Achard et al., 2008).”
Temperature stress and redox homeostasis in agricultural crops (2015, background): “However, the degradation of DELLAs is stimulated by gibberellins (Achard et al., 2008).”
Genomics of Cold Hardiness in Woody Plants (2014, background): “It was suggested that the reduced plant growth caused by the CBF-induced change in DELLA protein accumulation directly contributes to freezing tolerance (Achard et al., 2008; Thomashow, 2010).”
Plant hormone-mediated regulation of stress responses (2016, background): “Under stress conditions, this growth-restraining function of DELLAs helps to improve survival by diverting limited resources to defense responses.”