Construct design for efficient, effective and high-throughput gene silencing in plants.

By V. Wesley, C. Helliwell, Neil A. Smith, Ming-Bo Wang, D. Rouse, Qing Liu, P. Gooding, Surinder P. Singh, D. Abbott, Peter Stoutjesdijk and 4 more. The Plant Journal, 2001

1,621 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/7a5f9883440c084c759635d1e529a463d6b7a658

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

In Plant Science

Citations in the corpus

Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol (2007, methodology): “, RNA interference (RNAi) has been developed as an effective tool in plants and animal”
High frequency induction of RNA-mediated resistance against Cucumber mosaic virus using inverted repeat constructs (2004, background): “The spectacular silencing effect of hpRNA constructs has been demonstrated in protecting hosts from Potato virus Y and Barley yellow dwarf virus Smith et al. 2000; Wang et al. 2000; Wesley et al. 2001 .”
Gateway-compatible vectors for plant functional genomics and proteomics. (2006, methodology): “Destination vectors that make use of Gateway cloning in order to capture a given trigger RNA sequence in both the forward and reverse orientations have been designed by Helliwell and Waterhouse and are named ‘pHellsgate’ vectors (Helliwell and Waterhouse, 2003; Wes- ley et al., 2001) (Figure 1b).”
Achievements and Challenges in Legume Breeding for Pest and Disease Resistance (2015, background): “More recently, the development of gene silencing technologies using interference RNA (RNAi) or virus induced gene silencing (VIGS) have improved the efficiency of functional analysis of candidate genes (Wesley et al., 2001; Karchoo et al., 2008).”
Agrobacterium-mediated transformation of Nicotiana attenuata, a model ecological expression system (2002, background): “In particular, vectors will be required to increase the frequency of phenotypes resulting from reduced expression and we are currently exploring the utility of constructs containing inverted repeats of the gene of interest to this end, as has been pioneered by Waterhouse and associates ( Wesley et a”
Agrobacterium rhizogenes: recent developments and promising applications (2007, background): “The use of double-stranded RNA (dsRNA) to silence genes has been widely used in the plant research community (Fagard et al. 2000; Mette et al. 2000; Wesley et al. 2001; Waterhouse and Helliwell 2003).”
Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants (2005, methodology): “There are, however, aspects of RNA biochemistry, such as the effect of RNA processing, which may influence gene silencing, that remain poorly understood [13].”
Cloning Vectors for Rice (2009, methodology): “The gateway recombination system and multistep ligation methods are generally used for RNAi vector construction [21, 16, 23].”