Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: collaborative study.

By D. R. Mertens. Journal of AOAC International, 2002

2,823 citations recorded by the source corpus.

As an important constituent of animal feeds, fiber represents the portion of feeds that is bulky and difficult to digest. The neutral detergent fiber (NDF) method, developed over 30 years ago, is the method of choice for measuring total fiber in forages and other feeds. Several modifications that were made to improve its general applicability to all feeds and others developed in individual laboratories often resulted in variability among laboratories in measuring NDF. The amylase-treated NDF (aNDF) method, therefore, was developed as an accurate and precise method of measuring total insoluble fiber in feeds. A collaborative study was conducted to evaluate the repeatability and reproducibility of the aNDF method over the full range of animal feed materials. Twelve laboratories representing research, feed company, regulatory, and commercial feed testing laboratories analyzed 11 materials as blind duplicates. The materials represented feed matrixes, including animal products; high-protein, high-fat, and high-pectin feeds; oil seeds; grains; heated by-product feeds; and legume and grass hays and silages. Materials selected varied in chemical composition and contained 0-90% aNDF, 1-16% ash, 1-20% crude fat, 1-40% crude protein, and 0-50% starch. Correcting results for changes in blanks and reporting results as ash-free aNDF organic matter (aNDFom) improved the repeatability and reproducibility of results when aNDF was <25%. The within-laboratory repeatability standard deviation (Sr) for percentage aNDFom in feeds varied from 0.21 to 1.82 and among-laboratory reproducibility standard deviation (S(R)) varied from 0.37 to 2.24. The HORRAT was <2 for all materials except feed materials containing >10% fat. However, standard deviations of repeatability and reproducibility for feeds with >10% fat were similar to those of other materials. It is recommended that the aNDF method be accepted for Official First Action status.

Subfields: Food Science

View Paper: https://www.semanticscholar.org/paper/792b8f9b820959c08a4cfd68e4ea3579df1f2500

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Food Science

Citations in the corpus

Industrial hemp response to nitrogen, phosphorus, and potassium fertilization (2015, positive): “Neutral detergent fiber (NDF) was analyzed following Mertens (2002) with addition of heat-stable a-amylase, acid detergent fiber (ADF) was determined according to AOAC (1990), and acid detergent lignin (ADL) was quantified according to Robertson and Van Soest (1981) in the 74 biomass samples.”
Use of stomach tubing as an alternative to rumen cannulation to study ruminal fermentation and microbiota in sheep and goats (2014, positive): “The aNDF and ADF were determined as described by Mertens (2002) and the AOAC (2006; Official Method 973.”
Evaluation and application of the CPM Dairy Nutrition model (2007, methodology): “Several techniques for determining NDF have been developed and used (Hintz et al. 1996; Mertens 2002).”
Biochemical methane potential and biodegradability of complex organic substrates. (2011, positive): “Hemicellulose, cellulose, and lignin content were determined according to the neutral detergent fiber (NDF) and acid detergent fiber and lignin (ADF/ADL) analyses described by Mertens (2002) and Möller (2009), respectively.”