Extraction, purification and analysis of histones
By D. Shechter, Holger L. Dormann, C. D. Allis, S. Hake. Nature Protocols, 2007
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View Paper: https://www.semanticscholar.org/paper/78495700d9641f645c65e4e0927bb7de8681c71c
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Citations in the corpus
- Class I histone deacetylases (HDAC1–3) are histone lysine delactylases (2021, methodology): “Quantification of HDAC3-mediated histone delactylation in vitro To corroborate the results in peptide-based in vitro assays, we examined the delactylase activity of HDAC3 on histones by incubation of recombinant HDAC3 with acid-extracted histones from HeLa cells (54).”
- CBP-mediated acetylation of histone H3 lysine 27 antagonizes Drosophila Polycomb silencing (2009, methodology): “Histone extractions in acid or high salt buffer from embryos were carried out as described (Shechter et al., 2007).”
- Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes (2011, methodology): “After cell harvest, samples were subjected to histone enrichment as previously describe”
- Local states of chromatin compaction at transcription start sites control transcription levels (2021, methodology): “Acetic acid-urea-tritone X100 polyacrylamide gel electrophoresis (AUT-PAGE) was performed as described previously (44,45).”
- Virtual Ligand Screening of the p300/CBP Histone Acetyltransferase: Identification of a Selective Small Molecule Inhibitor (2010, background): “Loss of a positive charge upon lysine acetylation retards migration, producing a ladder of bands corresponding to integral changes in acetylation (Shechter et al., 2007).”
- Histone Recognition and Large-Scale Structural Analysis of the Human Bromodomain Family (2012, methodology): “Histones were extracted with high salt (Shechter et al., 2007) and incubated with recombinant biotinylated BRDs in the presence of (+)-JQ1 or ( )-JQ1 prior to purification on Strep-Tactin Sepharose beads.”