New bioactive metabolites produced by Colletotrichum sp., an endophytic fungus in Artemisia annua
By Hong Lu, W. Zou, J. Meng, Jun Hu, R. Tan. 2000
342 citations recorded by the source corpus.
In addition to ergosterol (I), 3 beta,5 alpha,6 beta-trihydroxyergosta-7,22-diene (II), 3 beta-hydroxy-ergosta-5-ene (III), 3-oxo-ergosta-4,6,8(14),22-tetraene (TV), 3 beta-hydroxy-5 alpha,8 alpha-epidioxy-ergosta-6,22-diene, 3 beta-hydroxy-5 alpha,8 alpha-epidioxy-ergosta-6,9(11),22-triene (VI) and 3-oxo-ergosta-4-ene (VII), a plant hormone indole-3-acetic acid (IAA) and three new antimicrobial metabolites were characterized from the culture of Colletotrichum sp., an endophyte isolated from inside the stem of Artemisia annua. The structures of the new metabolites were elucidated by a combination of spectroscopic methods (IR, MS, H-1 and C-13 NMR) as 6-isoprenylindole-3-carboxylic acid (1), 3 beta,5 alpha-dihydroxy-6 beta-acetoxy-ergosta-7,22 (2) and 3 beta,5 alpha-dihydroxy-6 beta-phenylacetyloxy-ergosta-7,22-diene (3), respectively. The compounds 1-3 and III-V inhibited the growth of all the tested bacteria (Bacillus subtilis, Staphylococcus aureus, Sarcina lutea and Pseudomonas sp.) with minimal inhibitory concentrations (MICs) ranging from 25 to 75 mu g/ml. Moreover, metabolites 2 and 3, together with the known sterols III and V, were inhibitory against the fungi Candida albicans and Aspergillus niger with MICs between 50 and 100 mu g/ml. At 200 mu g/ml, compounds 1-3, III and IV were shown to be fungistatic to the crop pathogenic fungi Gaeumannomyces graminis var, tritici, Rhizoctonia cerealis, Helminthosporium sativum and Phytophthora capisici. This is the first report on the endophytic fungus from A. annua and the bioactive metabolites thereof. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
Subfields: Cancer Research, Pharmacology
View Paper: https://www.semanticscholar.org/paper/774be9e2151aa272aa2db56c56752f4b81721061
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In Pharmacology
Citations in the corpus
- Biology of Endophytic Fungi (2012, background): “Lu et al. (2010) investigated the endophytic fungus Penicillium expansum, isolated from roots of the mangrove plant Excoecaria agallocha, and found it to produce”
- Bioprospecting for Microbial Endophytes and Their Natural Products (2003, background): “annua produced not only metabolites with activity against human-pathogenic fungi and bacteria but also metabolites that were fungistatic to plant-pathogenic fungi (42).”
- Promotion of plant growth by an auxin-producing isolate of the yeast Williopsis saturnus endophytic in maize (Zea mays L.) roots (2005, background): “Several endophytic bacteria (Bashan and Holguin 1997; Bastian et al. 1998), filamentous fungi ( Lu et al. 2000 ) and yeasts (Nakamura et al. 1991) have been reported to produce auxins in vitro.”
- Sterols in microorganisms (2002, background): “Several dihydroxylated sterols including 3b,5a-dihydroxy-6b-acetoxy-ergosta7,22-diene and 3b,5a-dihydroxy-6b-phenylacetyloxy-ergosta-7,22-diene from Colletotrichum sp., an endophytic fungus in Artemisia annua, have been shown to inhibit the growth of some bacteria (Lu et al. 2000).”
- Promotion of plant growth by phytohormone-producing endophytic microbes of sugar beet (2009, background): “Several endophytic bacteria (Harish et al. 2008; Sapak et al. 2008; Shin et al. 2007) and filamentous fungi (Lu et al. 2000) can produce auxins in vitro.”