Hydrogen peroxide acts as a signal molecule in the adventitious root formation of mung bean seedlings

By Shi-Weng Li, Lingui Xue, Shijian Xu, H. Feng, L. An. 2009

130 citations recorded by the source corpus.

Abstract Hydrogen peroxide (H 2 O 2 ), an active oxygen species, is widely generated in many biological systems and mediates various physiological and biochemical processes in plants. In this study we demonstrated that the exogenous H 2 O 2 was able to promote the formation and development of adventitious roots in mung bean seedlings. Treatments with 1–100 mM H 2 O 2 for 8–18 h significantly induced the formation and development of adventitious roots. Catalase (CAT) and ascorbic acid, which are H 2 O 2 scavengers or inhibitors, eliminated the adventitious root-promoting effects of exogenous H 2 O 2 . H 2 O 2 may have a downstream signaling function in the auxin signaling pathway and be involved in auxin-induced adventitious root formation. 2,3,5-Triiodobenzoic acid (TIBA), an inhibitor of auxin polar transport, strongly inhibited adventitious rooting of mung bean seedlings; however, the inhibiting effects of TIBA on adventitious rooting can be partially reversed by the exogenous IBA or H 2 O 2 . Diphenylene iodonium (DPI) strongly inhibits the activity of NADPH oxidase, which is one of the main sources of H 2 O 2 formation in plant cells. DPI treatment strongly inhibited the formation of adventitious roots in mung bean, but the inhibitory effects of DPI on rooting can be partially reversed by the exogenous H 2 O 2 or IBA. This indicates that the formation of adventitious roots was blocked once the generation of H 2 O 2 through NADPH oxidase was inhibited, and H 2 O 2 mediated the IBA-induced adventitious root formation. Furthermore, a rapid increase in the endogenous level of H 2 O 2 was detected during incubation with water 12–36 h after the primary root removal in mung bean seedlings. Three hours after the primary root removal, the generation of endogenous H 2 O 2 was markedly induced in IBA-treated seedlings in comparison with water-treated seedlings. This implies that IBA induced overproduction of H 2 O 2 in mung bean seedlings, and that IBA promoted adventitious root formation via a pathway involving H 2 O 2 . Results obtained suggest that H 2 O 2 may function as a signaling molecule involved in the formation and development of adventitious roots in mung bean seedlings.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/76947b850d290c53f8eb56297179d67a891a616a

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Citations in the corpus

• Exogenous hydrogen peroxide can enhance tolerance of wheat seedlings to salt stress (2011, background): “in rice, maize and mung bean seedlings (Prasad et al. 1994; Uchida et al. 2002; Li et al. 2009; Wang et al. 2010).”
• Physiological and Molecular Regulation of Adventitious Root Formation (2015, background): “H2O2 is also proposed to interact with auxin signaling possibly by decreasing peroxidase (POD) and ascorbate peroxidase (APX) activity during induction (Li et al., 2007; Li et al., 2009a; Li et al., 2009b).”