Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton

By A. Apprill, Sean P. McNally, R. Parsons, L. Weber. 2015

2,200 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/7624b4b863ddf62f56bdf8db22f25ead943f70d9

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In Ecology

In Molecular Biology

Citations in the corpus

American Gut: an Open Platform for Citizen Science Microbiome Research (2018, neutral): “Sequencing prior to August 2014 372 was done using the 515f/806r primer pair with the barcode on the reverse primer (50); 373 subsequent rounds were sequenced with the updated 515f/806rB primer pair with the barcode on 374 the forward read (51).”
Linking meta-omics to the kinetics of denitrification intermediates reveals pH-dependent causes of N2O emissions and nitrite accumulation in soil (2020, background): “7 the 16S, 515f and 806rB [49, 50], as detailed by the Earth Microbiome project 198 (http://www.”
Cultivating the Macroalgal Holobiont: Effects of Integrated Multi-Trophic Aquaculture on the Microbiome of Ulva rigida (Chlorophyta) (2020, methodology): “, 2011) and 806RB (5GGA CTA CNV GGG TWT CTA AT-3) (Apprill et al., 2015) and the sequencing was performed on an Illumina MiSeq platform to generate paired-end sequences of 300 bp in length.”
Evaluation of Filtration and DNA Extraction Methods for Environmental DNA Biodiversity Assessments across Multiple Trophic Levels (2017, methodology): “Both forward (515fB) and reverse (806rB) primers for the 16S rRNA gene, targeting microorganisms, were tagged yielding dual-indexed reads (Kozich et al., 2013; Apprill et al., 2015; Parada et al., 2016; Table 1).”
Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples (2018, background): “The primer pair 515F/806RB (Apprill et al. 2015) include unpublished modifications (e.”