Surface-Charge-Dependent Cell Localization and Cytotoxicity of Cerium Oxide Nanoparticles
By Atul Asati, S. Santra, C. Kaittanis, J. Perez. ACS Nano, 2010
653 citations recorded by the source corpus.
Cerium oxide nanoparticles (nanoceria) have shown great potential as antioxidant and radioprotective agents for applications in cancer therapy. Recently, various polymer-coated nanoceria preparations have been developed to improve their aqueous solubility and allow for surface functionalization of these nanoparticles. However, the interaction of polymer-coated nanoceria with cells, their uptake mechanism and subcellular localization are poorly understood. Herein, we engineered polymer-coated cerium oxide nanoparticles with different surface charge (positive, negative and neutral) and studied their internalization and toxicity in normal and cancer cell lines. Results showed that nanoceria with a positive or neutral charge enters most of the cell lines studied, while nanoceria with a negative charge internalizes mostly in the cancer cell lines. Moreover, upon entry into the cells, nanoceria is localized to different cell compartments (e.g. cytoplasm and lysosomes) depending on the nanoparticle's surface charge. The internalization and subcellular localization of nanoceria plays a key role in the nanoparticles’ cytotoxicity profile, exhibiting significant toxicity when they localize in the lysosomes of the cancer cells. In contrast, minimal toxicity is observed when they localize into the cytoplasm or do not enter the cells. Taken together, these results indicate that the differential surface-charge-dependent localization of nanoceria in normal and cancer cells plays a critical role in the nanoparticles’ toxicity profile.
Subfields: Biomedical Engineering, Materials Chemistry
View Paper: https://www.semanticscholar.org/paper/75ef3713211a7cd131d39e09b66cd46cb677e134
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In Materials Chemistry
Citations in the corpus
- The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles (2012, neutral): “Independent from the release of hydrolytic enzymes, a correlation of cytotoxicity and lysosomal localization has been described for CeO 2 NPs.(75) Anionic CeO 2 NPs”
- Dependence of Nanoparticle Toxicity on Their Physical and Chemical Properties (2018, background): “Specifically, positively charged and neutral NPs are absorbed by all cell types at the same rate, whereas negatively charged ones predominantly accumulate in tumor cells [93].”
- Techniques for physicochemical characterization of nanomaterials (2013, background): “Recent studies have shown improvement of cellular and lysosomeuptakes of positively-charged nanomaterials, compared with their neutral or negatively-charged counterparts (Asati et al., 2010; Baoum et al., 2010; Klesing et al., 2010; Liu et al., 2011; Luyts et al., 2013).”