Introduction to Isoform Sequencing Using Pacific Biosciences Technology (Iso-Seq)

By M. Gonzalez-Garay. 2016

95 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/741bafcf45692d5dc317feea02088d3af09242ee

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. Charlotte Soneson
2. Michael I. Love
3. Rob Patro
4. Mark D. Robinson
5. Luke Zappia
6. Lieven Clement
7. K. Van den Berge
8. Katharina M. Hembach

Citations in the corpus

• Using intron position conservation for homology-based gene prediction (2016, background): “Wet-lab experiments like RNA-seq (3) or Iso-seq (4) provide a wealth of information about transcripts including exon–intron boundaries and Untranslated region (UTRs), but are limited to those transcripts expressed under the studied conditions.”
• RNA Sequencing Data: Hitchhiker's Guide to Expression Analysis (2018, background): “In contrast, so­called third­generation , or long­read, sequencing technologies, led by Pacific Biosciences (PacBio) [202] and Oxford Nanopore Technologies (ONT), are able to generate reads that are much longer.”
• Opportunities and challenges in long-read sequencing data analysis (2020, background): “PacBio’s Iso-Seq3 [127, 128] is the most mature pipeline for long-read transcriptome analysis, allowing the assembly of full-length transcripts.”