Highly Efficient Generation of Human Hepatocyte–like Cells from Induced Pluripotent Stem Cells
By K. Si-Tayeb, Fallon K. Noto, M. Nagaoka, Jixuan Li, M. Battle, Christine B. Duris, P. North, S. Dalton, Stephen A. Duncan. Hepatology, 2010
1,229 citations recorded by the source corpus.
There exists a worldwide shortage of donor livers available for orthotropic liver transplantation and hepatocyte transplantation therapies. In addition to their therapeutic potential, primary human hepatocytes facilitate the study of molecular and genetic aspects of human hepatic disease and development and provide a platform for drug toxicity screens and identification of novel pharmaceuticals with potential to treat a wide array of metabolic diseases. The demand for human hepatocytes, therefore, heavily outweighs their availability. As an alternative to using donor livers as a source of primary hepatocytes, we explored the possibility of generating patient‐specific human hepatocytes from induced pluripotent stem (iPS) cells. Conclusion: We demonstrate that mouse iPS cells retain full potential for fetal liver development and describe a procedure that facilitates the efficient generation of highly differentiated human hepatocyte‐like cells from iPS cells that display key liver functions and can integrate into the hepatic parenchyma in vivo. (HEPATOLOGY 2010.)
Subfields: Genetics, Hepatology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/73ee7997dd85f7316123cdb325721e9f4d6e14a7
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Citations in the corpus
- In vitro generation of human pluripotent stem cell derived lung organoids (2015, background): “DOI: 10.7554/eLife.05098 16 of 25 (Si-Tayeb et al., 2009; Hrvatin et al., 2014).”
- Systematic Identification of Culture Conditions for Induction and Maintenance of Naive Human Pluripotency (2014, neutral): “For this purpose we subjected human ESCs derived from WIBR2 and WIBR3 primed cells in 5i/L/A to a five-step protocol for hepatic differentiation (Si-Tayeb et al., 2010).”
- Highly efficient miRNA-mediated reprogramming of mouse and human somatic cells to pluripotency (2011, neutral): “…cell biology, but also in the ability to generate patient-specific iPSC clones, which can then be further differentiated into the cell lineage of choice, including hematopoietic, cardiomyocyte, and hepatocyte cell lineages (Moretti et al., 2010a, 2010b; Raya et al., 2010; Si-Tayeb et al., 2010).”