Array-Based Electrical Detection of DNA with Nanoparticle Probes

By So-Jung Park, T. Taton, Chad A. Mirkin. Science, 2002

1,309 citations recorded by the source corpus.

A DNA array detection method is reported in which the binding of oligonucleotides functionalized with gold nanoparticles leads to conductivity changes associated with target-probe binding events. The binding events localize gold nanoparticles in an electrode gap; silver deposition facilitated by these nanoparticles bridges the gap and leads to readily measurable conductivity changes. An unusual salt concentration–dependent hybridization behavior associated with these nanoparticle probes was exploited to achieve selectivity without a thermal-stringency wash. Using this method, we have detected target DNA at concentrations as low as 500 femtomolar with a point mutation selectivity factor of ∼ 100,000:1.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/737cf9f0fb3a9faaf8a91e39b667b812cec74d7c

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Citations in the corpus

Electrochemical Immunosensors for Detection of Cancer Protein Biomarkers (2012, methodology): “The LBL approach yielded a 10-fold increase in the detection limit for the target DNA.”
Nanoparticles in tissue engineering: applications, challenges and prospects (2018, methodology): “156 Park et al 157 developed an electrical DNA detection method through binding of oligonucleotides with GNPs with a high accuracy.”
Noble Metal Nanoparticles for Biosensing Applications (2012, background): “In the case of the Mirkin’s group approach [156], the presence of a complementary target allowed the gold nanoprobes to form a sandwich hybridization with the probes”