Bacterial, Fungal, and Viral Disease Resistance Loci Mapped in a Recombinant Inbred Common Bean Population (`Dorado'/XAN 176)
By Phillip N. Miklas, R. Delorme, Valerie Stone, Mark J. Daly, J. Stavely, J. Steadman, M. J. Bassett, J. Beaver. 2000
115 citations recorded by the source corpus.
Understanding the genomic associations among disease resistance loci will facilitate breeding of multiple disease resistant cultivars. We constructed a genetic linkage map in common bean ( Phaseolus vulgaris L.) containing six genes and nine quantitative trait loci (QTL) comprising resistance to one bacterial, three fungal, and two viral pathogens of bean. The mapping population consisted of 79 F 5:7 recombinant inbred lines (RILs) derived from a 'Dorado'/XAN 176 hybridization. There were 147 randomly amplified polymorphic DNA (RAPD) markers, two sequence characterized amplified region (SCAR) markers, one intersimple sequence repeat (ISSR) marker, two seedcoat color genes R and V, the Asp gene conditioning seed brilliance, and two rust ( Uromyces appendiculatus var. appendiculatus (Pers.:Pers) Unger) resistance genes: one conditioning resistance to Races 53 and 54 and the other conditioning resistance to Race 108. These markers mapped across eleven linkage groups, one linked triad, and seven linked pairs for an overall map length of 930 cM (Kosambi). Genes conditioning resistance to anthracnose ( Co-2) (Colletotrichum lindemuthianum (Sacc. and Magnus) Lams.-Scrib.), bean rust (Ur-5), and bean common mosaic virus (I and bc-3) (BCMV) did not segregate in this population, but were mapped by inference using linked RAPD and SCAR markers identified in other populations. Nine previously reported quantitative trait loci (QTL) conditioning resistance to a variety of pathogens including common bacterial blight (Xanthomonas campestris pv. phaseoli (Smith) Dye), ashy stem blight (Macrophomina phaseolina (Tassi) Goid.), and bean golden mosaic virus (BGMV), were located across four linkage groups. Linkage among QTL for resistance to ashy stem blight, BGMV, and common bacterial blight on linkage group B7 and ashy stem blight, BGMV, and rust resistance loci on B4 will complicate breeding for combined resistance to all four pathogens in this population. opment of multiple disease resistant cultivars is often compli- cated by repulsion (trans) linkages among desired resistance genes and QTL used to control different pathogens in a particular production region (Kelly and Miklas, 1998). The development of an integrated map of resistance loci is just beginning, however, as many loci have yet to be mapped and the genomic relationships among several mapped loci are still unknown; thus, a comprehen-
Subfields: Plant Science
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Citations in the corpus
- QTL Conditioning Physiological Resistance and Avoidance to White Mold in Dry Bean (2001, background): “(Nodari et al., 1993; Miklas et al., 2000), and a closure and less biomass.”
- A major QTL for common bacterial blight resistance derives from the common bean great northern landrace cultivar Montana No.5 (2003, methodology): “…screened in the greenhouse, and a few resistant and susceptible tepary bean accessions including Tepary #4, were assayed for the SAP6 SCAR marker which is tightly linked with a QTL on linkage group B10 that conditions resistance to CBB that derives from GN#1 Sel 27 (Miklas et al., 2000a; 2000b).”
- Common bean breeding for resistance against biotic and abiotic stresses: From classical to MAS breeding (2006, background): “In fact, all three markers can be multiplexed in a single PCR reaction (Miklas et al., 2000b) to expedite MAS for combined resistance to CBB (Figure 2).”
- A Comprehensive Review of the Major Genes Conditioning Resistance to Anthracnose in Common Bean (2004, background): “Further confi rmation comes from Miklas et al. (2000a) who mapped the R gene to B8 and independently Gepts (1999) who also confi rmed that the C locus is located on B8.”