Activity-enhancing mutations in an E3 ubiquitin ligase identified by high-throughput mutagenesis

By Lea M. Starita, J. Pruneda, Russell S. Lo, Douglas M. Fowler, Helen J. Kim, Joseph B Hiatt, Jay Shendure, P. Brzovic, Stanley Fields, R. Klevit. 2013

197 citations recorded by the source corpus.

Subfields: Molecular Biology

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

Citations in the corpus

Measuring the activity of protein variants on a large scale using deep mutational scanning (2014, methodology): “In addition to enabling deep mutational scanning of longer regions 23 , subassembled libraries also provide internal replication, because each protein variant can be linked to more than one unique barcode.”
How Structural and Physicochemical Determinants Shape Sequence Constraints in a Functional Enzyme (2015, methodology): “These technologies have been applied to full scans of a few proteins in studies about tolerance to substitution and distribution of fitness effects [18–22] and to monitor directed evolution experiments [23,24].”
Massively Parallel Functional Analysis of BRCA1 RING Domain Variants (2015, methodology): “Communicating editor: S. K. Sharan 422 L. M. Starita et al. GENETICS Supporting Information http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.115.175802/-/DC1 Massively Parallel Functional Analysis of BRCA1 RING Domain Variants Lea M. Starita, David L. Young, Muhtadi Islam, Jacob O. Kitzman,”
A Comprehensive, High-Resolution Map of a Gene’s Fitness Landscape (2014, background): “Growth competition experiments or experiments in which alleles are enriched based on a threshold for function are the current state of the art (Fowler et al. 2010; Araya et al. 2012; Deng et al. 2012; McLaughlin et al. 2012; Schlinkmann et al. 2012; Whitehead et al. 2012; Roscoe et al. 2013; Starita”
Variational auto-encoding of protein sequences (2017, methodology): “We report the outcomes from training the model using the procedure described above on 5 protein families for which fitness measurements are publicly available [8, 9, 10, 11, 12].”