Base editing: precision chemistry on the genome and transcriptome of living cells

By H. Rees, David R. Liu. Nature reviews genetics, 2018

1,428 citations recorded by the source corpus.

RNA-guided programmable nucleases from CRISPR systems generate precise breaks in DNA or RNA at specified positions. In cells, this activity can lead to changes in DNA sequence or RNA transcript abundance. Base editing is a newer genome-editing approach that uses components from CRISPR systems together with other enzymes to directly install point mutations into cellular DNA or RNA without making double-stranded DNA breaks. DNA base editors comprise a catalytically disabled nuclease fused to a nucleobase deaminase enzyme and, in some cases, a DNA glycosylase inhibitor. RNA base editors achieve analogous changes using components that target RNA. Base editors directly convert one base or base pair into another, enabling the efficient installation of point mutations in non-dividing cells without generating excess undesired editing by-products. In this Review, we summarize base-editing strategies to generate specific and precise point mutations in genomic DNA and RNA, highlight recent developments that expand the scope, specificity, precision and in vivo delivery of base editors and discuss limitations and future directions of base editing for research and therapeutic applications. Genome editing through direct editing of bases holds promise for achieving precise genomic changes at single-nucleotide resolution while minimizing the occurrence of potentially mutagenic double-strand DNA breaks. In this Review, Rees and Liu provide a comprehensive account of the state of the art of base editing of DNA and RNA, including the progressive improvements to methodologies, understanding and avoiding unintended edits, cellular and organismal delivery of editing reagents and diverse applications in research and therapeutic settings.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/7288709af294b7d2e4a7f60f1a5fff74f0ffb6b6

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Citations in the corpus

• Evaluation and Minimization of Cas9-Independent Off-Target DNA Editing by Cytosine Base Editors (2020, neutral): “CBEs have achieved high levels of single-nucleotide polymorphism (SNP) conversion with low levels of indels in numerous cell types and organisms, including animal models of human genetic diseases 3–7 .”
• CRISPR Gene Therapy: Applications, Limitations, and Implications for the Future (2020, background): “Many features in an sgRNA determine specificity including the seed sequence (a 10–12 bp region proximal to PAM on 3′ of spacer sequence) (29, 53), GC content (54, 55), and modifications such as 5′ truncation of the sgRNA (56).”