Xanthogenate nucleic acid isolation from cultured and environmental cyanobacteria

By D. Tillett, B. Neilan. 2000

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Metagenomic profiling of Arctic microbial mat communities as nutrient scavenging and recycling systems (2010, methodology): “DNA was extracted in 800 mL of XS-buffer (1% potassium-ethyl-xanthogenate; 800 mmol L21 ammonium acetate; 20 mmol L21 ethylenediaminetetraacetic acid [EDTA]; 1% sodium dodecyl sulfate [SDS]; 100 mmol L21 Tris-HCl, pH 7.4; Tillet and Neilan 2000) and ground with a sterile micropestle.”
Vertical distributions of nitrogen-fixing phylotypes at Stn ALOHA in the oligotrophic North Pacific Ocean (2005, methodology): “The DNA pellets were washed once with 70% ethanol, air-dried, and then resuspended in 100 µl of TE buffer (Tillett & Neilan 2000).”
High rates of N2 fixation by unicellular diazotrophs in the oligotrophic Pacific Ocean (2004, methodology): “Nucleic acids were extracted with the protocol described by Tillett and Neila”
Experiments linking nitrogenase gene expression to nitrogen fixation in the North Pacific subtropical gyre (2007, methodology): “DNA was extracted using methods based on the xanthogenate method (Tillett and Neilan 2000), with the addition of 23 xanthogenate buffer to the samples stored in TE.”