Animal DNA in PCR reagents plagues ancient DNA research

By J. Leonard, Orin C. Shanks, M. Hofreiter, Eva Kreuz, L. Hodges, W. Ream, R. Wayne, R. Fleischer. 2007

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Trawl and eDNA assessment of marine fish diversity, seasonality, and relative abundance in coastal New Jersey, USA (2021, background): “Human and domestic animal ASVs were amplified from multiple field samples and negative controls, as commonly reported (Leonard et al., 2007; Port et al., 2016; Stoeckle et al., 2018); these were considered as of unknown origin.”
From the field to the laboratory: Controlling DNA contamination in human ancient DNA research in the high-throughput sequencing era (2017, unknown): “Laboratory consumables and reagents can be contaminated by human DNA during production in the manufacturing facility, before arriving into an aDNA laboratory (Champlot et al. 2010; Deguilloux et al. 2011; Leonard et al. 2007).”
Wild or domesticated: DNA analysis of ancient water buffalo remains from north China (2008, background): “…is unknown at this stage since BSA was replaced by pig-gelatin within the PCR reaction, but the incident raises legitimate concerns over the ancient DNA analysis of common domesticated species as DNA from their modern counterparts can be easily introduced into work areas (Leonard et al., 2007).”