Advancing Crop Transformation in the Era of Genome Editing[OPEN]

By F. Altpeter, Nathan M. Springer, L. Bartley, A. Blechl, T. Brutnell, V. Citovsky, Liza J. Conrad, S. Gelvin, D. Jackson, A. Kausch and 10 more. The Plant Cell, 2016

618 citations recorded by the source corpus.

Plant transformation has enabled fundamental insights into plant biology and revolutionized commercial agriculture. Unfortunately, for most crops, transformation and regeneration remain arduous even after more than 30 years of technological advances. Genome editing provides novel opportunities to enhance crop productivity but relies on genetic transformation and plant regeneration, which are bottlenecks in the process. Here, we review the state of plant transformation and point to innovations needed to enable genome editing in crops. Plant tissue culture methods need optimization and simplification for efficiency and minimization of time in culture. Currently, specialized facilities exist for crop transformation. Single-cell and robotic techniques should be developed for high-throughput genomic screens. Plant genes involved in developmental reprogramming, wound response, and/or homologous recombination should be used to boost the recovery of transformed plants. Engineering universal Agrobacterium tumefaciens strains and recruiting other microbes, such as Ensifer or Rhizobium, could facilitate delivery of DNA and proteins into plant cells. Synthetic biology should be employed for de novo design of transformation systems. Genome editing is a potential game-changer in crop genetics when plant transformation systems are optimized.

Subfields: Biotechnology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/6f0aa1a093d5606aee1b1e558b132af429d3938c

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

1. G. Hoerster
2. Ning Wang
3. L. Ryan
4. E. Wu
5. Ajith Anand
6. Bill Gordon-Kamm
7. K. McBride
8. T. Jones

Citations in the corpus

• Use of non-integrating Zm-Wus2 vectors to enhance maize transformation (2020, background): “…technologies for a small subset of plant species (such as maize), the availability of efficient transformation methods remains one of the remaining challenges for the plant transformation community, and represents the major impediment to genome editing in many crops (Altpeter et al. 2016).”
• Genome engineering for crop improvement and future agriculture. (2021, neutral): “The transformation and regeneration steps still represent bottlenecks for plant genome editing, as these processes must be optimized for each species and each plant variety, which is an arduous task for most elite varieties and wild species (Altpeter et al., 2016).”