Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil.

By F. Poly, L. J. Monrozier, R. Bally. Research in Microbiology, 2001

802 citations recorded by the source corpus.

Subfields: Ecology, Health, Toxicology and Mutagenesis, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/6eedb193d0685e446589a6dde2e2654f4fa64adb

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Citations in the corpus

Methylocystis rosea sp. nov., a novel methanotrophic bacterium from Arctic wetland soil, Svalbard, Norway (78° N) (2006, positive): “The presence of nifH (Poly et al., 2001) indicates that strain SV97T actively fixes nitrogen (Auman et al., 2001).”
Diverse culturable diazotrophic endophytic bacteria from Poaceae plants show cross-colonization and plant growth promotion in wheat (2017, methodology): “…om P oa ce ae pl an ts E nd op hy te co m m un ity Pl an tp ar t D G G E ba nd (A cc es si on nu m be r) C lo se ly re la te d se qu en ce in N C B I (A cc es si on nu m be r) % Se qu en ce id en tit y (n um be r of ba se s) a Is ol at io n so ur ce b Ta xo no m ic gr ou p To ta le nd op hy te s R…”
Plant growth-promoting rhizobacteria and root system functioning (2013, background): “In this section, a focus is put on the regulation of the expression of PGPR plant-beneficial properties by biotic factors occurring in the rhizosphere.”
Diazotrophic diversity, nifH gene expression and nitrogenase activity in a rice paddy field in Fujian, China (2009, methodology): “In the first PCR reaction, a 370 base pair fragment of the nifH gene was amplified using the PCR primers PolF and PolR (Poly et al. 2001).”
Biogeographic patterns of microbial association networks in paddy soil within Eastern China (2020, background): “, 2012) for ITS genes and PolF/PolR (Poly et al., 2001) for nifH genes.”
Molecular detection and quantification of nifH gene sequences in the rhizosphere of sorghum (Sorghum bicolor) sown with two levels of nitrogen fertilizer (2009, methodology): “Putative diazotrophs belonging to the alpha, beta and gammaproteobacteria, Cyanobacteria and Firmicutes have already been found in soil and rhizosphere samples (Widmer et al., 1999; Poly et al., 2001a; Coelho et al., 2008).”
Influence of corn, switchgrass, and prairie cropping systems on soil microbial communities in the upper Midwest of the United States (2016, methodology): “In a more recent study, Gaby & Buckley (2012) reported that no nifH primers were comprehensive for the known nifH genes and that the primers developed by Poly et al. (2001) do exclude certain groups.”
Methylobacter tundripaludum sp. nov., a methane-oxidizing bacterium from Arctic wetland soil on the Svalbard islands, Norway (78° N) (2006, methodology): “SV96 was positive for the nifH gene by PCR (Poly et al., 2001).”
FunGene: the functional gene pipeline and repository (2013, methodology): “The genomic DNA was mixed together and ampliﬁed using the Poly primers (Poly et al., 2001) with one barcode (Wang et al., 2013)”
Nitrogenase gene diversity and microbial community structure: a cross-system comparison. (2003, methodology): “Using DGGE, it was found that the composition of the diazotroph community varied as a function of soil type on a range of scales (Poly et al., 2001b).”