Assessment of purity of rice hybrids using microsatellite and STS markers

By J. Yashitola, T. Thirumurugan, R. Sundaram, M. K. Naseerullah, M. Ramesha, N. Sarma, R. V. Sonti. 2002

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Marker-assisted selection: an approach for precision plant breeding in the twenty-first century (2008, methodology): “In hybrid rice, SSR and STS markers were used to confirm purity, which was considerably simpler than the standard ‘grow-out tests’ that involve growing the plant to maturity and assessing morphological and floral characteristics (Yashitola et al. 2002).”
Identification of informative SSR markers capable of distinguishing hybrid rice parental lines and their utilization in seed purity assessment (2008, methodology): “The genotype inferred from the marker proWle was compared with the phenotype at maturity to verify the results derived from marker analysis with GOT.”
Application of SSR markers for molecular characterization of hybrid parents and purity assessment of ICPH 2438 hybrid of pigeonpea [Cajanus cajan (L.) Millspaugh] (2010, background): “Although some studies reported the suitability of even one marker for hybrid purity assessment tests (Yashitola et al. 2002, Mishra et al. 2003, Nandakumar et al. 2004), Sundaram et al. (2008) demonstrated the superiority of using two to three markers in a multiplex over single-marker system.”
Molecular fingerprinting of hybrids and assessment of genetic purity of hybrid seeds in rice using microsatellite markers (2004, background): “Molecular markers have the potential in achieving this goal (Jena & Pandey, 1999; Yashitola et al., 2002).”