Replication of Human Noroviruses in Stem Cell-Derived Human Enteroids
By K. Ettayebi, S. Crawford, Kosuke Murakami, J. Broughman, Umesh C. Karandikar, Victoria R. Tenge, F. Neill, S. Blutt, Xi-Lei Zeng, Lin Qu and 7 more. Science, 2016
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The major barrier to research and development of effective interventions for human noroviruses (HuNoVs) has been the lack of a robust and reproducible in vitro cultivation system. HuNoVs are the leading cause of gastroenteritis worldwide. We report the successful cultivation of multiple HuNoV strains in enterocytes in stem cell–derived, nontransformed human intestinal enteroid monolayer cultures. Bile, a critical factor of the intestinal milieu, is required for strain-dependent HuNoV replication. Lack of appropriate histoblood group antigen expression in intestinal cells restricts virus replication, and infectivity is abrogated by inactivation (e.g., irradiation, heating) and serum neutralization. This culture system recapitulates the human intestinal epithelium, permits human host-pathogen studies of previously noncultivatable pathogens, and allows the assessment of methods to prevent and treat HuNoV infections.
Subfields: Animal Science and Zoology, Genetics, Infectious Diseases
View Paper: https://www.semanticscholar.org/paper/6cbb00ac38bd7d2f9b465264777aa208948ac42f
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Citations in the corpus
- The digestive system is a potential route of 2019-nCov infection: a bioinformatics analysis based on single-cell transcriptomes (2020, neutral): “intestinal secretion and activated enteric nervous system[36-38].”
- SARS-CoV-2 productively infects human gut enterocytes (2020, background): “Of note, hSIOs have allowed the first in vitro culturing of Norovirus (24).”