A proposal for validation of antibodies

By Mathias Uhlen, Anita E. Bandrowski, Steven A. Carr, A. Edwards, Jan Ellenberg, E. Lundberg, David L. Rimm, H. Rodriguez, Tara Hiltke, Michael Snyder and 1 more. Nature Methods, 2016

562 citations recorded by the source corpus.

We convened an ad hoc International Working Group for Antibody Validation in order to formulate the best approaches for validating antibodies used in common research applications and to provide guidelines that ensure antibody reproducibility. We recommend five conceptual 'pillars' for antibody validation to be used in an application-specific manner.

Subfields: Immunology, Molecular Biology, Radiology, Nuclear Medicine and Imaging

View Paper: https://www.semanticscholar.org/paper/6c3b10bc4d00c676b4a25718630d083157989eb2

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In Molecular Biology

1. Cheng Zhang
2. Adil Mardinoglu
3. Mathias Uhlen
4. Linn Fagerberg

Citations in the corpus

• A subcellular map of the human proteome (2017, methodology): “” Locations obtained the score “validated” if the antibody was validated according to oneof the validation “pillars” proposed by an international working group (36) as suitable for IF: (i) genetic methods using”
• Reproducibility: Designing a rigorous microscopy experiment: Validating methods and avoiding bias (2019, unknown): “There are currently no standardized requirements for validation of specificity of commercial antibodies (Couchman, 2009; Uhlen et al., 2016).”