A complete Cannabis chromosome assembly and adaptive admixture for elevated cannabidiol (CBD) content
By Christopher J. Grassa, Jonathan P Wenger, Clemon J. Dabney, Shane G. Poplawski, S. T. Motley, Todd P. Michael, C. Schwartz, G. Weiblen. bioRxiv, 2018
88 citations recorded by the source corpus.
Cannabis has been cultivated for millennia with distinct cultivars providing either fiber and grain or tetrahydrocannabinol. Recent demand for cannabidiol rather than tetrahydrocannabinol has favored the breeding of admixed cultivars with extremely high cannabidiol content. Despite several draft Cannabis genomes, the genomic structure of cannabinoid synthase loci has remained elusive. A genetic map derived from a tetrahydrocannabinol/cannabidiol segregating population and a complete chromosome assembly from a high-cannabidiol cultivar together resolve the linkage of cannabidiolic and tetrahydrocannabinolic acid synthase gene clusters which are associated with transposable elements. High-cannabidiol cultivars appear to have been generated by integrating hemp-type cannabidiolic acid synthase gene clusters into a background of marijuana-type cannabis. Quantitative trait locus mapping suggests that overall drug potency, however, is associated with other genomic regions needing additional study. Summary A complete chromosome assembly and an ultra-high-density linkage map together identify the genetic mechanism responsible for the ratio of tetrahydrocannabinol (THC) to cannabidiol (CBD) in Cannabis cultivars, allowing paradigms for the evolution and inheritance of drug potency to be evaluated.
Subfields: Endocrinology, Diabetes and Metabolism, Molecular Biology, Pharmacology
View Paper: https://www.semanticscholar.org/paper/6c31b2274c0852293339a83a3b35e35a66c27590
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In Molecular Biology
Citations in the corpus
- Development and validation of genetic markers for sex and cannabinoid chemotype in Cannabis sativa L. (2020, background): “It has previously been demonstrated that the factors affecting cannabinoid chemotype are not linked to total cannabinoid content (Grassa et al., 2018).”