Application and Analysis of the Folin Ciocalteu Method for the Determination of the Total Phenolic Content from Limonium Brasiliense L.
By Andressa Blainski, G. C. Lopes, J. D. de Mello. Molecules, 2013
884 citations recorded by the source corpus.
Limonium brasiliense is a common plant on the southern coast of Brazil. The roots are traditionally used for treatment of premenstrual syndrome, menstrual disturbances and genito-urinary infections. Pharmaceutical preparations obtained from its roots and used for these purposes were marketed in Brazil in the 1980s and 1990s. Currently, the Brazilian Drug Agency (National Health Surveillance Agency, ANVISA) has canceled the registration of these products, and their use was discontinued because of a lack of studies to characterize the plant raw material and ensure the effectiveness and safety of its use. The aim of the present study was to develop and validate an analytical method to determine the content of total polyphenols (TP) in an extract from L. brasiliense roots, by the UV/Vis spectrophotometric method. L. brasiliense roots were extracted in acetone:water (7:3, v/v-10% w/v). The crude extract was used to develop a method for TP assay. The method was validated according to national and international guidelines. The optimum conditions for analysis time, wavelength, and standard substance were 30 min, 760 nm, and pyrogallol, respectively. Under these conditions, validation by UV/Vis spectrophotometry proved the method to be linear, specific, precise, accurate, reproducible, robust, and easy to perform. This methodology complies with the requirements for analytical application and to ensure the reliability of the results.
Subfields: Complementary and alternative medicine, Food Science, Plant Science
View Paper: https://www.semanticscholar.org/paper/6c2ddf3fe19aa176650c690974476b8922bb1b20
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In Complementary and alternative medicine
In Food Science
In Plant Science
- Ó. Vicente
- Marina Fuertes
- I. Munteanu
- Francisco Sanchez
- Constantin Apetrei
- M. A. Hassan
- M. Boscaiu
- F. Rejali
Citations in the corpus
- Effects of Salt and Water Stress on Plant Growth and on Accumulation of Osmolytes and Antioxidant Compounds in Cherry Tomato (2015, methodology): “Total phenolic compounds were quantified by reaction with the Folin-Ciocalteu reagent, according to Blainski et al. (2013).”
- Arbuscular Mycorrhiza and Plant Growth-promoting Bacteria Alleviate Drought Stress in Walnut (2019, methodology): “Total phenolic content of leaves was determined using Folin–Ciocalteu reagent (Blainski et al., 2013).”
- Analytical Methods Used in Determining Antioxidant Activity: A Review (2021, methodology): “At present, the Folin–Ciocalteu test is commercially available from several important commercial societies, so this method is widely used to quantify polyphenols in plantderived extracts [55], as well as in foods and beverages [9].”