Cellular Response of Pea Plants to Cadmium Toxicity: Cross Talk between Reactive Oxygen Species, Nitric Oxide, and Calcium1[W][OA]

By M. Rodríguez-Serrano, M. Romero‐Puertas, Diana M. Pazmiño, P. Testillano, M. Risueño, L. A. Del Río, L. Sandalio. Plant Physiology, 2009

611 citations recorded by the source corpus.

Cadmium (Cd) toxicity has been widely studied in different plant species; however, the mechanism involved in its toxicity as well as the cell response against the metal have not been well established. In this work, using pea (Pisum sativum) plants, we studied the effect of Cd on antioxidants, reactive oxygen species (ROS), and nitric oxide (NO) metabolism of leaves using different cellular, molecular, and biochemical approaches. The growth of pea plants with 50 μm CdCl2 affected differentially the expression of superoxide dismutase (SOD) isozymes at both transcriptional and posttranscriptional levels, giving rise to a SOD activity reduction. The copper/zinc-SOD down-regulation was apparently due to the calcium (Ca) deficiency induced by the heavy metal. In these circumstances, the overproduction of the ROS hydrogen peroxide and superoxide could be observed in vivo by confocal laser microscopy, mainly associated with vascular tissue, epidermis, and mesophyll cells, and the production of superoxide radicals was prevented by exogenous Ca. On the other hand, the NO synthase-dependent NO production was strongly depressed by Cd, and treatment with Ca prevented this effect. Under these conditions, the pathogen-related proteins PrP4A and chitinase and the heat shock protein 71.2, were up-regulated, probably to protect cells against damages induced by Cd. The regulation of these proteins could be mediated by jasmonic acid and ethylene, whose contents increased by Cd treatment. A model is proposed for the cellular response to long-term Cd exposure consisting of cross talk between Ca, ROS, and NO.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/6c25c29b04bcb91c10119e167451639ddccc0b9b

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Citations in the corpus

Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. (2010, background): “Cd induced decrease in Ca lead to Cu/Zn SOD down-regulation which resulted in the overproduction of the H 2 O 2 and O 2 À in Pisum sativum [35].”
Heavy Metal Tolerance in Plants: Role of Transcriptomics, Proteomics, Metabolomics, and Ionomics (2016, background): “In the SA signaling under heavy metal stress, several signaling molecules such as nitric oxide (NO), H2O2, Ca+2, etc. and their interactions have been reported (Rodríguez-Serrano et al., 2009; Xu et al., 2014).”
Abiotic and Biotic Stress Response Crosstalk in Plants (2011, background): “In pea plants, the cellular response to long-term cadminum exposure consists of crosstalk between Ca2+- and ROS- activated pathways and signaling mediated by nitric oxide (NO) (Rodriguez-Serrano et al., 2009).”
Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “peroxide (H2O2), and their interactions, have been identified as being potentially involved in the cellular responses of plants to heavy metal toxicity (Rodríguez-Serrano et al., 2009).”
Systemic mitigation of salt stress by hydrogen peroxide and sodium nitroprusside in strawberry plants via transcriptional regulation of enzymatic and non-enzymatic antioxidants (2014, background): “In addition, he employment of ‘omics’ approaches supported the previously eported cross-talk between H2O2 and NO signaling upon abiotic tress or stress-free conditions (Rodriguez-Serrano et al., 2009; anou et al., 2012).”
The molecular mechanism of zinc and cadmium stress response in plants (2012, background): “Excess Cd can lead to Ca deficiency, which results in the downregulation of antioxidant enzymes CAT and CSD [186, 187]; as a result, less ROS are scavenged.”
Physiological and ultra-structural changes in Brassica napus seedlings induced by cadmium stress (2014, background): “In different crops, exposure to Cd reduces plant size, the number of lateral roots (Sandalio et al. 2001, Rodriguez-Serrano et al. 2009), chlorophyll content, and photosynthetic rate, whereas elevates malondialdehyde (MDA) content, and superoxide dismutase (SOD) and peroxidase (POD) activities…”
Cadmium stress in plants: A critical review of the effects, mechanisms, and tolerance strategies (2020, background): “…pathogenesis-related gene 1 [NPR1], salicylic acid-binding protein 3 [SABP3], the transcription factor TGA1, and the NADPH oxidase AtRBOHD) responsible for signaling and regulating Cd stress (Astier et al., 2012; Chmielowska-Bąk et al., 2014; Rodriguez-Serrano et al., 2009; Spoel & Loake, 2011).”
Exogenous nitric oxide improves antioxidative capacity and reduces auxin degradation in roots of Medicago truncatula seedlings under cadmium stress (2009, background): “Cd2+ treatment reduced the accumulation of Ca2+. Rodríguez-Serrano et al. (2009) found that exogenous supply of Ca2+ decreased the accumulation of Cd2+ in pea plants, suggesting that the competition between both elements for the same transporters.”