Improved solubility of TEV protease by directed evolution.

By S. van den Berg, Per-Åke Löfdahl, T. Härd, H. Berglund. 2006

323 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology, Radiology, Nuclear Medicine and Imaging

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In Molecular Biology

1. Marianne Rooman
2. Yves Dehouck
3. Dimitri Gilis
4. Sahand Hormoz
5. Jean Marc Kwasigroch

Citations in the corpus

• Intracellular Detection and Evolution of Site-Specific Proteases Using a Genetic Selection System (2012, background): “The need for a sensitive, rapid, and high-throughput analysis of mutant enzymes isolated in the process of the directed evolution experiment, combined with limited and unpredictable solubilities of enzymes derived from TEV-Pr [32], restricted the utility of a monophasic implementation of the kinetic”
• Enhancing the stability and solubility of TEV protease using in silico design (2007, background): “The first is directed evolution experiments, where the protein is subjected to random mutagenesis and then screened for the chosen property (Roodveldt et al. 2005; van den Berg et al. 2006).”