A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

By Martin Jinek, K. Chylinski, Ines Fonfara, Michael H. Hauer, Jennifer A. Doudna, E. Charpentier. 2012

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In Molecular Biology

Citations in the corpus

Evolutionary-scale prediction of atomic level protein structure with a language model (2022, background): “[31] Martin Jinek, Krzysztof Chylinski, Ines Fonfara, Michael Hauer, Jennifer A.”
Evolved Cas9 variants with broad PAM compatibility and high DNA specificity (2018, positive): “2 and Extended Data Figs 5–7) may reflect more extensive requirements for DNA cleavage than DNA binding 27,32 , or differences in the chromatin state of plasmid (Fig.”
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing (2014, neutral): “” Nature biotechnology 32 (6): 569-576.”
Genome Engineering Using the CRISPR Cas9 System (2019, background): “This system functions through three steps [9,12].”
Large‐scale image‐based screening and profiling of cellular phenotypes (2017, background): “Although RNAi and CRISPR/ Cas9 were first discovered in plants and bacteria, respectively, they can also be used to knockdown, knockout, or even enhance the expressions of specific genes in other species, including human, at the cellular (43–45) or organismal (46,47) levels.”
Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System (2015, background): “coli, the CRISPR-Cas9 system has been demonstrated to apply allelic exchange with efficiency as high as 65% 14% (24) and to control gene expression via a nuclease-deficient Cas9 protein (34, 35).”
Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells (2014, positive): “To target particular genomic loci in eukaryotic cells, the type II CRISPR-Cas system from Streptococcus pyogenes has been adapted so that it requires the nuclease Cas9 and one sgRNA 6–9 .”
Genome engineering for crop improvement and future agriculture. (2021, neutral): “The Cas9 protein requires an additional RNA molecule known as a trans -acting crRNA (tracrRNA), which can be artiﬁcially fused with the corresponding crRNA to form a sgRNA (Jinek et al., 2012).”
Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients. (2013, methodology): “The system utilizes the type II prokaryotic CRISPR/Cas9 adaptive immune system and targets the Cas9 nuclease by a 20 nt guide sequence cloned upstream of a 50-NGG ‘‘protospacer adjacent motif’’ (PAM) (Jinek et al., 2012).”