A structural analysis of M protein in coronavirus assembly and morphology
By B. Neuman, Gabriella Kiss, Andreas H. Kunding, D. Bhella, M. Baksh, S. Connelly, B. Droese, Joseph P. Klaus, S. Makino, S. Sawicki and 5 more. Journal of Structural Biology, 2010
742 citations recorded by the source corpus.
The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into workshops where virus and host factors come together to make new virus particles. We investigated how M structure and organization is related to virus shape and size using cryo-electron microscopy, tomography and statistical analysis. We present evidence that suggests M can adopt two conformations and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation, presumably through interactions with M. These findings provide insight into how M protein functions to promote virus assembly.
Subfields: Animal Science and Zoology, Infectious Diseases
View Paper: https://www.semanticscholar.org/paper/67e477ca127575683a98bb69ccd6bae775b09e61
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Citations in the corpus
- Structures of Human Antibodies Bound to SARS-CoV-2 Spike Reveal Common Epitopes and Recurrent Features of Antibodies (2020, positive): “To address whether inter-spike crosslinking by anti-SARSCoV-2 IgGs could occur, we modeled adjacent S proteins on a virion membrane assuming a minimum inter-spike separation distance of 15 nm, as observed from cryo-electron tomography analyses of SARS-CoV and other coronaviruses (Neuman et al., 2011”
- Emerging coronaviruses: Genome structure, replication, and pathogenesis (2020, positive): “The M protein has three transmembrane domains and it shapes the virions, promotes membrane curvature, and binds to the nucleocapsid.(52,53) The E protein plays a role in virus assembly and release, and it involved in viral pathogenesis.”
- Molecular Architecture of the SARS-CoV-2 Virus (2020, positive): “Distinguished from the SARS-CoV virus, which was estimated to possess an average of ∼50-100 spikes per virion (Neuman et al., 2011), the SARS-CoV-2 virus possesses approximately half of the prefusion S and occasionally some postfusion S.”
- SARS-CoV-2 (COVID-19) by the numbers (2020, methodology): “(Neuman et al., 2011): "Our model predicts ~90 spikes per”
- Coronaviruses: An Overview of Their Replication and Pathogenesis (2015, background): “studies suggest the M protein exists as a dimer in the virion, and may adopt two different conformations, allowing it to promote membrane curvature as well as to bind to the nucleocapsid [ 13 ].”