Controlled vesicle deformation and lysis by single oscillating bubbles

By P. Marmottant, Sascha Hilgenfeldt. Nature, 2003

831 citations recorded by the source corpus.

The ability of collapsing (cavitating) bubbles to focus and concentrate energy, forces and stresses is at the root of phenomena such as cavitation damage, sonochemistry or sonoluminescence. In a biomedical context, ultrasound-driven microbubbles have been used to enhance contrast in ultrasonic images. The observation of bubble-enhanced sonoporation—acoustically induced rupture of membranes—has also opened up intriguing possibilities for the therapeutic application of sonoporation as an alternative to cell-wall permeation techniques such as electroporation and particle guns. However, these pioneering experiments have not been able to pinpoint the mechanism by which the violently collapsing bubble opens pores or larger holes in membranes. Here we present an experiment in which gentle (linear) bubble oscillations are sufficient to achieve rupture of lipid membranes. In this regime, the bubble dynamics and the ensuing sonoporation can be accurately controlled. The use of microbubbles as focusing agents makes acoustics on the micrometre scale (microacoustics) a viable tool, with possible applications in cell manipulation and cell-wall permeation as well as in microfluidic devices.

Subfields: Biomedical Engineering, Electrical and Electronic Engineering, Materials Chemistry

View Paper: https://www.semanticscholar.org/paper/67289addadc4b5ed09e7c01ff6f26ccf3fa1b6e6

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Citations in the corpus

Ultrasonic drug delivery – a general review (2004, background): “At high amplitudes, the associated shear forces are capable of shearing open red cells [10] and synthetic vesicles such as liposomes [7].”
Understanding ultrasound induced sonoporation: definitions and underlying mechanisms. (2014, background): “In this regard, it has been shown that microstreaming, generated by oscillations at low acoustic pressures, was responsible for the rupture of lipid vesicles [47] (Fig.”
Microfluidic sorting in an optical lattice (2003, methodology): “These microcapsules are an ultrasound contrast agent that can be used to locally permeate cell membranes (a technique known as sonoporatio”
Microfluidics: Fluid physics at the nanoliter scale (2005, methodology): “44, and has been used to lyse vesicles Marmottant and Hilgenfeldt, 2003 and to enhance mixing in cell capture Liu, Yang, et al., 2002 and DNA hybridization applications Liu, Lenigk, et al., Rev. Mod.”
Cavitation microstreaming patterns in single and multiple bubble systems (2007, methodology): “Rooney (1989) observed that the shearing stresses due to cavitation microstreaming caused haemolysis of erythrocytes, supporting the observations made by Marmottant & Hilgenfeldt (2003).”
Bubble Dynamics in Soft and Biological Matter (2019, background): “(e) Acoustic streaming around a bubble in contact with a wall (side view) (Marmottant & Hilgenfeldt 2003).”