Self-Assembled Nanoparticle Dimer Antennas for Plasmonic-Enhanced Single-Molecule Fluorescence Detection at Micromolar Concentrations

By D. Punj, Raju Regmi, Alexis Devilez, Robin Plauchu, S. Moparthi, B. Stout, N. Bonod, Hervé Rigneault, J. Wenger. 2015

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Plasmonic antennas offer extremely promising strategies to enhance single molecule fluorescence sensing and breach the limitations set by diffraction. However, the technical difficulty and limited availability of top-down nanofabrication techniques enabling nanometer gap sizes are limiting the impact of plasmonic antennas for biochemical and biophysical applications. Here we demonstrate the effectiveness of self-assembled nanoparticle gap antennas to enhance single molecule fluorescence detection at high concentrations. For a dimer of 80 nm gold nanoparticles with 6 nm gap, we isolate detection volumes down to 70 zL (equivalent to λ3/3600) and achieve 600-fold fluorescence enhancement, microsecond transit time, and operation of fluorescence correlation spectroscopy at concentrations exceeding 10 μM. We quantify the near-field detection volume and the fluorescence enhancement for different self-assembled nanoantenna designs using fluorescence correlation spectroscopy. The combination of the fabrication sim...

Subfields: Biomedical Engineering, Electronic, Optical and Magnetic Materials, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/660f1e07b44a04a6f3d1c2b85eb71d29b69eb875