Effect of different culture conditions and inducers on production of laccase by a basidiomycete fungal isolate Pleurotus ostreatus HP-1 under solid state fermentation
By Hardik Patel, A. Gupte, Shilpa Gupte. BioResources, 2009
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The production of laccase by an indigenous strain of Pleurotus ostreatus HP-1 was studied on solid state fermentation. Culture parameters such as type and concentration of substrate, inoculum size, moisture content, pH, surfactant presence, temperature, and nitrogen source were optimized by conventional “one factor at a time” methodology. A maximum laccase yield of 3952 U g-1 of dry substrate optimized was obtained with wheat straw as substrate with five agar plugs as the inoculum, 60% moisture content, pH 5.0, surfactant concentration 0.015 gl-1, and nitrogen source (combination of L-asparagine and NH4NO3 at 10 mM concentration each) at incubation temperature 28oC. Enhancement in laccase activity was achieved with the use of various aromatic inducers and copper sulphate. Highest laccase activity of 14189 U g-1 of dry substrate was achieved using 0.28 mM copper sulphate under optimized conditions. Thus, the indigenous isolate seems to be a potential producer of laccase using SSF and can be exploited for further biotechnological applications. The process also promises economic utilization and value addition of agro-residues.
Subfields: Biotechnology, Pharmacology, Plant Science
View Paper: https://www.semanticscholar.org/paper/65b914186644eeea995479ada8df16ce1f224155
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Citations in the corpus
- Biological pretreatment of lignocellulosic biomass--An overview. (2016, background): “Patel et al. (2009) reported that laccase production is significantly affected by change in pH of the medium.”
- Optimization of physical and nutritional factors for synthesis of lignin degrading enzymes by a novel strain of Trametes vericolor (2011, background): “On the other hand, higher inoculum size increases spore density, causing faster depletion of available nutrients (Galhaup et al. 2002; Patel et al. 2009).”
- Biological pretreatment of lignocelluloses with white-rot fungi and its applications: A review (2011, methodology): “Lac activity using P. pulmonarius decreases when moisture content increases from 60% to 80% (Patel et al. 2009b).”