Osmotic adjustment in transgenic citrus rootstock Carrizo citrange (Citrus sinensis Osb. x Poncirus trifoliata L. Raf.) overproducing proline

By H. B. C. Molinari, C. J. Marur, J. C. B. Filho, A. K. Kobayashi, Marcos Pileggi, R. L. Júnior, L. Pereira, L. Vieira. 2004

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Genetic engineering of chickpea (Cicer arietinum L.) with the P5CSF129A gene for osmoregulation with implications on drought tolerance (2009, background): “Indeed, osmolyte accumulation (OA) in plant cells has been shown to result in a decrease in the cell osmotic potential and thus in maintenance of water absorption and cell turgor pressure, which might contribute to sustaining physiological processes, such as stomatal opening, photosynthesis, and exp”
Enhanced proline accumulation and salt stress tolerance of transgenic indica rice by over-expressing P5CSF129A gene (2009, methodology): “... of the medium used, nutritional supplements, culture conditions prior to and during inoculation, duration of co-cultivation, virulence of Agrobacterium strain, concentration and composition of the bacteriostatic agent used, duration of selection and concentration of antibiotic selection marker, ”
New plant breeding techniques. State-of-the-art and prospects for commercial development (2011, background): “Inverted Repeat (IR) constructs seem to be the most effective (Mette et al., 2000; Muskens et al., 2000).”