The Chlamydomonas sourcebook

By E. H. Harris. 2009

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Metal bioremediation by CrMTP4 over-expressing Chlamydomonas reinhardtii in comparison to natural wastewater-tolerant microalgae strains (2017, methodology): “Strains were grown photoheterotrophically in batch culture in Tris–acetate–phosphate (TAP) medium at pH 7 [27] in 200 ml glass flasks on an orbital shaker rotating at 2 Hz or in 50 ml Nunc flasks, at 25°C under cool-white fluorescent lights (150 μmol m s) with a 16-h:8-h light:dark regime.”
Cultivation of Green Algae Chlorella sp. in Different Wastewaters from Municipal Wastewater Treatment Plant (2010, methodology): “It was preserved in Tris–acetate–phosphorus [13] media containing the following chemicals: NH4Cl 400 mg/L, MgSO4·7H2O 100 mg/L, CaCl2·2H2O 50 mg/L, K2HPO4 108 mg/L, KH2PO4 56 mg/L, Tris (hydroxymethyl)aminomethane 2,420 mg/L, glacial acetic acid 1 mL, and trace elements solution 1 mL. Trace elements”
Radiation characteristics of Botryococcus braunii, Chlorococcum littorale, and Chlorella sp. used for CO2 fixation and biofuel production (2009, methodology): “Finally, the chlorophyll a and chlorophyll b concentrations were determined for each strain using the ethanol extraction method developed by Wintermans and De Mots [66].”