Membrane Protein Assembly into Nanodiscs
By Timothy H. Bayburt, S. G. Sligar. FEBS Letters, 2009
723 citations recorded by the source corpus.
Nanodiscs are soluble nanoscale phospholipid bilayers which can self‐assemble integral membrane proteins for biophysical, enzymatic or structural investigations. This means for rendering membrane proteins soluble at the single molecule level offers advantages over liposomes or detergent micelles in terms of size, stability, ability to add genetically modifiable features to the Nanodisc structure and ready access to both sides of the phospholipid bilayer domain. Thus the Nanodisc system provides a novel platform for understanding membrane protein function. We provide an overview of the Nanodisc approach and document through several examples many of the applications to the study of the structure and function of integral membrane proteins.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/64391a02fedb7a5e48f794ce0460af5ea3e9df24
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In Molecular Biology
Citations in the corpus
- Advances in methods for atomic resolution macromolecular structure determination (2020, background): “Nanodiscs—lipid bilayer disks bound by encircling protein molecules 43 —are providing new and fruitful routes to analyzing membrane proteins by cryo-EM 44–47 .”
- The Amyloid-β Oligomer Hypothesis: Beginning of the Third Decade (2018, background): “These docking stations organize multiple membrane proteins [8], including neurotransmitter receptors linked to A O-induced neuron damage [243].”