Comparison of the bligh and dyer and folch methods for total lipid determination in a broad range of marine tissue

By S. Iverson, Shelley L C Lang, M. H. Cooper. Lipids, 2001

709 citations recorded by the source corpus.

Subfields: Aquatic Science, Molecular Biology, Nutrition and Dietetics

View Paper: https://www.semanticscholar.org/paper/620c9a306d0c5472c730ab6971b7cf294995865b

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Aquatic Science

1. S. Iverson
2. Ian Stirling
3. G. Thiemann
4. A. Springer
5. W. Bowen
6. A. Kitaysky
7. S. Budge
8. P. Szpak

In Molecular Biology

1. P. Szpak
2. W. Bowen
3. S. Iverson
4. Ian Stirling
5. G. Thiemann
6. S. Budge
7. J. P. Turner
8. A. Springer

In Nutrition and Dietetics

1. P. Szpak
2. Ian Stirling
3. G. Thiemann
4. S. Budge
5. J. P. Turner
6. A. Springer
7. A. Kitaysky
8. H. Koopman

Citations in the corpus

• Evaluating quantitative fatty acid signature analysis (QFASA) using harbour seals Phoca vitulina richardsi in captive feeding studies (2008, methodology): “Lipids were extracted from homogenized harbour seal blubber and whole prey samples using a modified Folch technique (Folch et al. 1957, Iverson et al. 2001).”
• Climate regime shifts and reorganization of fish communities: the essential fatty acid limitation hypothesis (2006, methodology): “Following Iverson et al. (2001), we corrected lipid data derived using the Bligh & Dyer method for comparison with the Folch method, using the equation: log (Folch) = 0.047 + 1.122 × log (Bligh & Dyer) (1) Because we tested the null hypothesis of no relationship between lipid content and population…”
• VARIABILITY IN THE BLUBBER FATTY ACID COMPOSITION OF RINGED SEALS (PHOCA HISPIDA) ACROSS THE CANADIAN ARCTIC (2007, methodology): “Lipid was extracted and isolated from each biopsy subsample according to Iverson et al. (2001) and FA methyl esters (FAME) prepared from each lipid sample using an acidic catalyst (the Hilditch method; Hilditch and Williams 1964, Thiemann et al. 2004).”
• Fish Processing Wastes as a Potential Source of Proteins, Amino Acidsand Oils: A Critical Review (2013, methodology): “[150] extracted lipids from fish tissues using the Bligh and Dyer extraction method.”
• Fish bone chemistry and ultrastructure: implications for taphonomy and stable isotope analysis (2011, methodology): “Iverson et al. (2001) have demonstrated that for fish tissues with >2% lipids, the Bligh and Dyer method was relatively ineffective, and in samples with high lipid contents, up to 50% of lipids were not successfully extracted.”
• Biochemical composition of the Atlantic bonito Sarda sarda from the Aegean Sea (eastern Mediterranean Sea) in different stages of sexual maturity (2006, methodology): “(1957), which does not underestimate the lipid content of tissues containing >2% lipid (Iverson et al., 2001).”
• STUDYING TROPHIC ECOLOGY IN MARINE ECOSYSTEMS USING FATTY ACIDS: A PRIMER ON ANALYSIS AND INTERPRETATION (2006, methodology): “…specific; for example, the most useful and abundant dietary FA and isomers can differ between the North Pacific, North Atlantic, and tropical Pacific (Iverson et al. 1997, S. J. Iverson1) as well as with fresh-water or terrestrial ecosystems (e.g., Smith et al. 1996, Iverson et al. 2001b).”
• POLAR BEAR DIETS AND ARCTIC MARINE FOOD WEBS: INSIGHTS FROM FATTY ACID ANALYSIS (2008, methodology): “Lipid was quantitatively extracted from each adiposetissue sample according to Iverson et al. (2001) and FA methyl esters (FAME) were prepared using H2SO4 as a catalyst (Thiemann et al. 2004).”
• How to account for the lipid effect on carbon stable‐isotope ratio (δ13C): sample treatment effects and model bias (2008, methodology): “when d(13)C is used as a tracer for primary carbon sources, and hence alternative and more efficient extraction methods (Folch et al., 1957; Iverson et al., 2001) should be tested and applied.”
• Trophic ecology of Sargassum-associated fishes in the Gulf of Mexico determined from stable isotopes and fatty acids (2006, methodology): “Lipids were first extracted in duplicate aliquots in chloroform:methanol (2:1 by volume) similar to the method of Iverson et al. (2001), and fatty acid methyl esters were prepared following Iverson et al. (1992).”
• Indicators of Energetic Status in Juvenile Coho Salmon and Chinook Salmon (2005, background): “This may have been due to taxonomic differences (ie, salmonids versus percids), differences in lipid and protein composition, or to differences in the procedures used to determine the concentrations of proximate constituents ( Iverson et al. 2001 ).”
• QUANTITATIVE FATTY ACID SIGNATURE ANALYSIS: A NEW METHOD OF ESTIMATING PREDATOR DIETS (2004, methodology): “…Wamberg et al. 1992, Colby et al. 1993, Pond et al. 1995, Raclot et al. 1998), the degree to which plants have been consumed by terrestrial carnivores (Iverson and Oftedal 1992, Iverson et al. 2001b), and changes in the diets of pinnipeds (Iverson 1993, Iverson et al. 1997a, Kirsch et al. 2000).”
• Seabirds as indicators of food web structure and ecosystem variability: qualitative and quantitative diet analyses using fatty acids (2007, methodology): “Lipid was quantitatively extracted from seabird fat samples and homogenates of each individual prey according to Iverson et al. (2001).”
• Tracing aquatic food webs using fatty acids: from qualitative indicators to quantitative determination (2009, background): “Methods for isolation, preparation, and analysis of lipids and FA have been extensively reviewed elsewhere (Christie 1982 ; Ackman 1986, 2002 ; Parrish 1999 ; Iverson et al. 2001a ; Budge et al. 2006 ; and references therein).”
• On the Use of Stable Isotopes in Trophic Ecology (2011, methodology): “We characterized lipid extraction methods according to criteria listed in Smedes (1999), Manirakiza et al. (2001), and Iverson et al. (2001).”