Sample preparation and scanning protocol for computerised analysis of root length and diameter

By Tjeerd Joris Bouma, K. L. Nielsen, B. Koutstaal. Plant and Soil, 2004

366 citations recorded by the source corpus.

Subfields: Environmental Chemistry, Environmental Engineering, Plant Science

View Paper: https://www.semanticscholar.org/paper/617afe8f296a1bd3cd60f448b6af6a9685630a48

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Citations in the corpus

A combination of humic substances and Herbaspirillum seropedicae inoculation enhances the growth of maize (Zea mays L.) (2013, methodology): “The fresh weight of roots and shoots were measured and the superficial root area was estimated in three replications by Delta-T scan software (Bouma et al. 2000).”
A handbook of protocols for standardised and easy measurement of plant functional traits worldwide (2003, methodology): “More on methods: Böhm (1979) (general root methods); Fitter (1996) (general appearance of roots); Bouma et al. (2000) (root length and diameter).”
QTL Analysis of Adventitious Root Formation in Common Bean under Contrasting Phosphorus Availability (2006, methodology): “Scanned images were analyzed with WinRhizo Pro (Re´gent Instruments, Que´bec, Canada), adjusted as described by Bouma et al. (2000) .”
Quantifying root system quality of nursery seedlings and relationship to outplanting performance (2005, methodology): “Further development of new technologies, such as root scanning equipment, will lead to faster and more accurate non-destructive assessment of seedling root morphological quality (Rigney and Kranzler 1997; Bouma et al. 2000; Costa et al. 2001).”
The mysterious root length (2006, neutral): “transformation threshold settings (Bouma et al. 2000).”
Root traits as tools for creating phosphorus efficient crop varieties (2004, background): “This may be partly due to that even modern image analysis protocols (Bouma et al., 2000) for measuring root diameter are prone to errors.”
Linking fine root traits to maximum potential growth rate among 11 mature temperate tree species (2004, methodology): “matic threshold (brightness) method appropriate for each sample (automatic methods specific for pale, normal or dark roots; Bouma, Nielsen & Koutstaal 2000 ).”
Plant root traits affecting the resistance of soils to concentrated flow erosion (2012, methodology): “Root traits were measured using WinRHIZO PRO (version 2003b, Regent Instrument, Quebec, Canada) following the protocol described in Bouma et al. (2000). The roots were stained with blue methylene (5 g L) in order to increase contrast and then scanned at a resolution of 400 dpi.”
Fine root demography and morphology in response to soil resources availability among xeric and mesic sandhill tree species (2002, methodology): “After calibrating with delta-t scan image standards for length and thickness, images were scanned using Hewlett-Packard’s precision scan pro software (Hewlett-Packard, Palo Alto, CA, USA), with settings of 200 for brightness and 400 dpi for resolution ( Bouma, Nielsen & Koutstaal 2000 ).”