Remote Excitation of Neuronal Circuits Using Low-Intensity, Low-Frequency Ultrasound
By W. Tyler, Yusuf Tufail, M. Finsterwald, Monica L. Tauchmann, Emily J. Olson, Cassondra Majestic. PLoS ONE, 2008
668 citations recorded by the source corpus.
Possessing the ability to noninvasively elicit brain circuit activity yields immense experimental and therapeutic power. Most currently employed neurostimulation methods rely on the somewhat invasive use of stimulating electrodes or photon-emitting devices. Due to its ability to noninvasively propagate through bone and other tissues in a focused manner, the implementation of ultrasound (US) represents a compelling alternative approach to current neuromodulation strategies. Here, we investigated the influence of low-intensity, low-frequency ultrasound (LILFU) on neuronal activity. By transmitting US waveforms through hippocampal slice cultures and ex vivo mouse brains, we determined LILFU is capable of remotely and noninvasively exciting neurons and network activity. Our results illustrate that LILFU can stimulate electrical activity in neurons by activating voltage-gated sodium channels, as well as voltage-gated calcium channels. The LILFU-induced changes in neuronal activity were sufficient to trigger SNARE-mediated exocytosis and synaptic transmission in hippocampal circuits. Because LILFU can stimulate electrical activity and calcium signaling in neurons as well as central synaptic transmission we conclude US provides a powerful tool for remotely modulating brain circuit activity.
Subfields: Biomedical Engineering, Cellular and Molecular Neuroscience
View Paper: https://www.semanticscholar.org/paper/6155e5465aeab81eb3b965ab38fa6b1b71909d3d
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In Biomedical Engineering
In Cellular and Molecular Neuroscience
Citations in the corpus
- Transcranial pulsed ultrasound stimulates intact brain circuits. (2010, background): “In hippocampal slices, we previously reported low-intensity US ( 300 mW/cm2), low-frequency US ( 0.65 MHz) is capable of stimulating action potentials and synaptic transmission (Tyler et al., 2008).”
- Focused ultrasound modulates region-specific brain activity (2011, positive): “More recently, reversible excitation of the motor cortex has been demonstrated in the excised rodent brain tissue (Tyler et al., 2008) and live rodents (Tufail et al., 2010).”