Proteomic and Metabolic Elucidation of Solar-Powered Biomanufacturing by Bio-Abiotic Hybrid System

By Rutan Zhang, Yingjiao He, Jia Yi, Lijuan Zhang, Chengpin Shen, Shujuan Liu, Lifeng Liu, Baohong Liu, Liang Qiao. Chem, 2020

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Summary Highly efficient inorganic light absorbers combined with highly specific biocatalysts, i.e., chemolithoautotrophic microbes, have recently been proposed for solar-powered biomanufacturing to pave the way in artificial photosynthesis. In this work, we studied the global protein and metabolite changes of a Moorella thermoacetica-CdS (cadmium sulfide quantum dots) hybrid system during light harvesting. The Wood-Ljungdahl pathway (WLP) that has been considered as the main approach for CO2 fixation was found to be highly activated by CdS. Targeted metabolite quantification revealed that energy-metabolism-associated glycolysis and the tricarboxylic acid (TCA) cycle were also activated. Therefore, we propose an energy-conservation scheme to the M. thermoacetica-CdS hybrid system, i.e., glycolysis and the TCA cycle along with the oxidation of acetyl-coenzyme A (CoA) and NADH, in addition to the well-documented chemiosmotic proton gradient-driven ATP production by ATPase. We expect that the study can be helpful to guide the design of effective and biocompatible photo-biocatalytic systems in the future.

Subfields: Environmental Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/60ebb2ae85cc80902be382ee7e44f09609c4d280